| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 541 | Lachnospira eligens | TTGGCGTTGAGGTGCTTCT | AGCCAGTCAGCCTTGCAA | 59.85 | 59.48 | 163 | 49 |
100.00%
|
50.00%
|
| Primer 542 | Lachnospira eligens | TTGGCGTTGAGGTGCTTCT | AAGCCAGTCAGCCTTGCA | 59.85 | 59.48 | 164 | 49 |
100.00%
|
50.00%
|
| Primer 543 | Lachnospira eligens | TGGCGTGTCAGGTGATTCA | ACCTGCGAACCCTCTTCA | 59.25 | 58.11 | 159 | 49 |
100.00%
|
100.00%
|
| Primer 544 | Lachnospira eligens | GGAAATGGCGTGTCAGGTG | ACCTGCGAACCCTCTTCA | 59.12 | 58.11 | 164 | 49 |
100.00%
|
100.00%
|
| Primer 545 | Lachnospira eligens | ATGTACTTGCAGAGCAGGCA | CCCCATCGAGGTTCTTCCAG | 59.67 | 59.82 | 261 | 49 |
100.00%
|
100.00%
|
| Primer 546 | Lachnospira eligens | GCAACAAACTGCGGAGGA | ATTGCCACAGCCACAGCA | 58.26 | 60.20 | 180 | 49 |
100.00%
|
100.00%
|
| Primer 547 | Lachnospira eligens | ACACCCATTTCCACCGTTGT | CGTCTGCGGAACTGTCTCT | 60.11 | 59.42 | 160 | 49 |
100.00%
|
100.00%
|
| Primer 548 | Lachnospira eligens | ACACCCATTTCCACCGTTGT | TCGTCTGCGGAACTGTCT | 60.11 | 58.24 | 161 | 49 |
100.00%
|
100.00%
|
| Primer 549 | Lachnospira eligens | AGGTGCATCGCCAATCCA | AGATGCGCACCGACATCA | 59.32 | 59.42 | 180 | 49 |
100.00%
|
100.00%
|
| Primer 550 | Lachnospira eligens | TCGGTTGTAGCGGTTGAGG | GTCTTCAACCTCAACGTGCA | 59.71 | 58.70 | 273 | 49 |
100.00%
|
100.00%
|