| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Lactobacillus apis | AAAGTGCGCACGTTCTGC | AACGTCTCGTGCAGTTGC | 59.67 | 58.68 | 191 | 168 |
100.00%
|
100.00%
|
| Primer 2 | Lactobacillus apis | AAAGTGCGCACGTTCTGC | AGGGTAACGTCTCGTGCAG | 59.67 | 59.41 | 196 | 168 |
100.00%
|
100.00%
|
| Primer 3 | Lactobacillus apis | AAAGTGCGCACGTTCTGC | AGGGCACAAGGGTAACGTC | 59.67 | 59.63 | 204 | 158 |
100.00%
|
100.00%
|
| Primer 4 | Lactobacillus apis | AAAGTGCGCACGTTCTGC | GGGTAACGTCTCGTGCAGT | 59.67 | 59.71 | 195 | 143 |
100.00%
|
100.00%
|
| Primer 5 | Lactobacillus apis | AAAGTGCGCACGTTCTGC | TGCACGTAGGAGTTCACCC | 59.67 | 59.33 | 164 | 140 |
100.00%
|
100.00%
|
| Primer 6 | Lactobacillus apis | TCCGAGTGAGCTGTCTGGA | TCTCGGGTCATGGGTCACT | 59.93 | 59.92 | 196 | 138 |
100.00%
|
100.00%
|
| Primer 7 | Lactobacillus apis | CCGGAATTTGCCACAGAGG | ATCACGCTTTGTCGCAACT | 58.82 | 58.38 | 225 | 138 |
100.00%
|
100.00%
|
| Primer 8 | Lactobacillus apis | AAAGTGCGCACGTTCTGC | AGGGTAACGTCTCGTGCA | 59.67 | 58.23 | 196 | 138 |
100.00%
|
100.00%
|
| Primer 9 | Lactobacillus apis | ATTGGAAACGCGACCGCA | TGCCTGTCCATGGGAAAACA | 60.67 | 59.81 | 297 | 137 |
100.00%
|
100.00%
|
| Primer 10 | Lactobacillus apis | AAAGTGCGCACGTTCTGC | GCACGTAGGAGTTCACCCA | 59.67 | 59.33 | 163 | 135 |
100.00%
|
100.00%
|