| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 181 | Lactobacillus apis | TTTGAGCCAGGATGCCGT | AGCACCGTGCCAATGCT | 59.24 | 59.93 | 168 | 90 |
100.00%
|
100.00%
|
| Primer 182 | Lactobacillus apis | CGATTCGCGTCCAAGTGATG | ACGCTTTTGTCCAGCTGC | 59.70 | 58.97 | 190 | 89 |
100.00%
|
100.00%
|
| Primer 183 | Lactobacillus apis | CGATTCGCGTCCAAGTGATG | GACGCTTTTGTCCAGCTGC | 59.70 | 60.08 | 191 | 89 |
100.00%
|
100.00%
|
| Primer 184 | Lactobacillus apis | AGCGTGGTGACTTAGCTGC | ATTGGAGCTGCATCGCCT | 60.37 | 59.41 | 208 | 89 |
100.00%
|
100.00%
|
| Primer 185 | Lactobacillus apis | TAGGCAAGCGTGGTGACT | ATTGGAGCTGCATCGCCT | 58.53 | 59.41 | 214 | 89 |
100.00%
|
100.00%
|
| Primer 186 | Lactobacillus apis | GCAAGCGTGGTGACTTAGC | ATTGGAGCTGCATCGCCT | 59.50 | 59.41 | 211 | 89 |
100.00%
|
100.00%
|
| Primer 187 | Lactobacillus apis | GCGTGGTGACTTAGCTGCA | ATTGGAGCTGCATCGCCT | 60.67 | 59.41 | 207 | 89 |
100.00%
|
100.00%
|
| Primer 188 | Lactobacillus apis | TAGGCAAGCGTGGTGACTT | ATTGGAGCTGCATCGCCT | 59.25 | 59.41 | 214 | 89 |
100.00%
|
100.00%
|
| Primer 189 | Lactobacillus apis | ATCGAATTAAAGCGCTCGACG | GACGAAGGGACCTGTTGCA | 59.48 | 59.93 | 235 | 88 |
100.00%
|
100.00%
|
| Primer 190 | Lactobacillus apis | ACTCAGCGTTTACGGCTGA | TCAAGCACGCGTTGGTCA | 59.34 | 60.20 | 194 | 88 |
100.00%
|
100.00%
|