| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Lactobacillus ultunensis | GGAGCAATTGTCGCTAGTTGG | TCCGCAAAATGTTCAGCAGC | 59.60 | 60.04 | 197 | 165 |
100.00%
|
50.00%
|
| Primer 2 | Lactobacillus ultunensis | GGCCATTGCTTTTGTACCAGG | ACCGCTAGCACCAAAGACC | 60.07 | 60.00 | 256 | 160 |
100.00%
|
50.00%
|
| Primer 3 | Lactobacillus ultunensis | GGCCTTGATGCTGCAACAG | ACAGCGTGTGTCATCCAGG | 59.79 | 60.00 | 248 | 148 |
100.00%
|
50.00%
|
| Primer 4 | Lactobacillus ultunensis | TGATGGCTTTGGGCTTAGGAG | ACGAAGTGCACGAATCTTGT | 60.06 | 58.13 | 179 | 148 |
100.00%
|
50.00%
|
| Primer 5 | Lactobacillus ultunensis | CGTGGTTCGTCAACTGCAG | TTTCTCCCTGCGGTGAACC | 59.44 | 59.93 | 197 | 148 |
100.00%
|
50.00%
|
| Primer 6 | Lactobacillus ultunensis | GGTCGCCTATTCTCCGATGG | GGAACGTCTTCAGGCTTGC | 60.04 | 59.13 | 266 | 146 |
100.00%
|
50.00%
|
| Primer 7 | Lactobacillus ultunensis | ACGTTATGTGCACACGATTGC | GCAACCTCCCCTTCATCTCC | 60.14 | 60.11 | 167 | 146 |
100.00%
|
50.00%
|
| Primer 8 | Lactobacillus ultunensis | ACGTTATGTGCACACGATTGC | CCACATTCTGAACGGCTGG | 60.14 | 58.83 | 217 | 146 |
100.00%
|
50.00%
|
| Primer 9 | Lactobacillus ultunensis | ACAATGCTTCCTTTTGGGGAT | AGGTGGATGAGACGCAATACC | 58.09 | 59.86 | 155 | 141 |
100.00%
|
50.00%
|
| Primer 10 | Lactobacillus ultunensis | ACAATGCTTCCTTTTGGGGAT | GGTGGATGAGACGCAATACC | 58.09 | 58.42 | 154 | 141 |
100.00%
|
50.00%
|