| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Lactococcus cremoris | ACCGGTGAACTTGGAGCTG | TGGCAAATCAGCTCCGAGC | 59.93 | 60.75 | 246 | 143 |
100.00%
|
100.00%
|
| Primer 2 | Lactococcus cremoris | ACCGGTGAACTTGGAGCTG | TCAGCTCCGAGCATTTCCC | 59.93 | 59.78 | 239 | 140 |
100.00%
|
100.00%
|
| Primer 3 | Lactococcus cremoris | TGGAGGAACGACTGGTGGA | GCTTCGGCAATTTCGGCA | 60.15 | 59.43 | 258 | 127 |
100.00%
|
100.00%
|
| Primer 4 | Lactococcus cremoris | GCCGACATCATTGCCAAGC | GCTTCGGCAATTTCGGCA | 60.23 | 59.43 | 239 | 127 |
100.00%
|
100.00%
|
| Primer 5 | Lactococcus cremoris | TGGAGCCGACATCATTGCC | GCTTCGGCAATTTCGGCA | 60.45 | 59.43 | 243 | 127 |
100.00%
|
100.00%
|
| Primer 6 | Lactococcus cremoris | CTGGTGGAGCCGACATCAT | GCTTCGGCAATTTCGGCA | 59.48 | 59.43 | 247 | 127 |
100.00%
|
100.00%
|
| Primer 7 | Lactococcus cremoris | GGGTTGCTGTTGGACGAAC | GCTTCGGCAATTTCGGCA | 59.35 | 59.43 | 202 | 127 |
100.00%
|
100.00%
|
| Primer 8 | Lactococcus cremoris | GGAGCCGACATCATTGCCA | GCTTCGGCAATTTCGGCA | 60.45 | 59.43 | 242 | 124 |
100.00%
|
100.00%
|
| Primer 9 | Lactococcus cremoris | GGGGTTGCTGTTGGACGAA | GCTTCGGCAATTTCGGCA | 60.53 | 59.43 | 203 | 117 |
100.00%
|
100.00%
|
| Primer 10 | Lactococcus cremoris | ACCGGTGAACTTGGAGCTG | TCTGGCAAATCAGCTCCGAG | 59.93 | 60.11 | 248 | 115 |
100.00%
|
100.00%
|