| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1591 | Leptospira biflexa | AAGCGAAACGACCCGAGTT | AGCCACCAGCCACTCATTC | 59.93 | 60.00 | 172 | 78 |
100.00%
|
100.00%
|
| Primer 1592 | Leptospira biflexa | ATGCTCTGAACCCAAGGCC | TCGACCAGGTCCGCTTCTA | 60.00 | 60.00 | 286 | 78 |
100.00%
|
100.00%
|
| Primer 1593 | Leptospira biflexa | TCCGAAAAGGCACCCTTCC | TCGACCAGGTCCGCTTCTA | 59.93 | 60.00 | 256 | 78 |
100.00%
|
100.00%
|
| Primer 1594 | Leptospira biflexa | CCCTTCCACCCGACAAACA | TCGACCAGGTCCGCTTCTA | 59.85 | 60.00 | 244 | 78 |
100.00%
|
100.00%
|
| Primer 1595 | Leptospira biflexa | CACCCTTCCACCCGACAAA | TCGACCAGGTCCGCTTCTA | 59.85 | 60.00 | 246 | 78 |
100.00%
|
100.00%
|
| Primer 1596 | Leptospira biflexa | ACCCTTCCACCCGACAAAC | TCGACCAGGTCCGCTTCTA | 59.85 | 60.00 | 245 | 78 |
100.00%
|
100.00%
|
| Primer 1597 | Leptospira biflexa | AAAAGGCACCCTTCCACCC | TCGACCAGGTCCGCTTCTA | 60.15 | 60.00 | 252 | 78 |
100.00%
|
100.00%
|
| Primer 1598 | Leptospira biflexa | GTCCAAGGGTATGGCCAACA | TTGCCTTCCGGTCCTGTT | 59.96 | 58.75 | 167 | 78 |
100.00%
|
100.00%
|
| Primer 1599 | Leptospira biflexa | TGTCCAAGGGTATGGCCAAC | TTGCCTTCCGGTCCTGTT | 59.96 | 58.75 | 168 | 78 |
100.00%
|
100.00%
|
| Primer 1600 | Leptospira biflexa | CGGAAGTGGCATCCCGTTA | CCGAGTCAATGCCCTTGCT | 59.78 | 60.38 | 168 | 78 |
100.00%
|
100.00%
|