| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2181 | Leptospira biflexa | AAGTTGCTCGAGGACCTGC | GTGTTCAACCCGACTCATCCA | 60.00 | 60.27 | 257 | 68 |
100.00%
|
100.00%
|
| Primer 2182 | Leptospira biflexa | GCACGGAGACACGATCCAT | ACCGGAATGGATGACTGCG | 59.86 | 60.15 | 184 | 68 |
100.00%
|
100.00%
|
| Primer 2183 | Leptospira biflexa | AGTGCACGGAGACACGATC | ACCGGAATGGATGACTGCG | 59.79 | 60.15 | 187 | 68 |
100.00%
|
100.00%
|
| Primer 2184 | Leptospira biflexa | ACCAAGTGCACGGAGACA | ACCGGAATGGATGACTGCG | 58.77 | 60.15 | 191 | 68 |
100.00%
|
100.00%
|
| Primer 2185 | Leptospira biflexa | TGCGGACTACGGCAAAGT | TGACCAGAATAGGCATGCCG | 59.26 | 60.18 | 183 | 68 |
100.00%
|
100.00%
|
| Primer 2186 | Leptospira biflexa | GAGGAAGACAACGCCTCGT | GCCGAGAAGCTCTTGCACT | 59.71 | 60.38 | 287 | 68 |
100.00%
|
100.00%
|
| Primer 2187 | Leptospira biflexa | AACCTGGGCAGTCATCGT | TCGAAGCGCAATTCCCACT | 58.52 | 60.00 | 230 | 68 |
100.00%
|
100.00%
|
| Primer 2188 | Leptospira biflexa | AGAACCTGGGCAGTCATCG | TCGAAGCGCAATTCCCACT | 59.40 | 60.00 | 232 | 68 |
100.00%
|
100.00%
|
| Primer 2189 | Leptospira biflexa | GAACCTGGGCAGTCATCGT | TCGAAGCGCAATTCCCAC | 59.70 | 58.43 | 231 | 68 |
100.00%
|
100.00%
|
| Primer 2190 | Leptospira biflexa | TCCCACCGCACAAGCATT | CCAAGCAGAACACCATCCCA | 59.88 | 60.25 | 199 | 68 |
100.00%
|
100.00%
|