| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3011 | Leptospira biflexa | TCGTCCCGATCCTGACCAA | TCCCGTCAGAGACACCCAA | 60.30 | 60.15 | 219 | 52 |
100.00%
|
100.00%
|
| Primer 3012 | Leptospira biflexa | ATCCGAGCACTTGGCCAA | TCCGCAGATGTTCCGATCC | 59.24 | 59.56 | 221 | 52 |
100.00%
|
100.00%
|
| Primer 3013 | Leptospira biflexa | TCGATGGGTTGGGAAGCAA | GGCGATGACACCAAGGGAT | 59.24 | 59.78 | 228 | 52 |
100.00%
|
100.00%
|
| Primer 3014 | Leptospira biflexa | CCCAACGCACCTGTGGATA | AGAGGCGAGTGGGGAAATG | 59.70 | 59.40 | 231 | 52 |
100.00%
|
100.00%
|
| Primer 3015 | Leptospira biflexa | CCCAACGCACCTGTGGATA | GGCGAGTGGGGAAATGGTA | 59.70 | 59.39 | 228 | 52 |
100.00%
|
100.00%
|
| Primer 3016 | Leptospira biflexa | TGGGAGAAGGTGGGGACAA | ATCGCCTTTCCACGTCCA | 60.07 | 58.93 | 177 | 52 |
100.00%
|
100.00%
|
| Primer 3017 | Leptospira biflexa | AACCCTTCCAAACAGGTGTGA | TGCCAAAATCGAACCGCAA | 59.71 | 58.97 | 161 | 52 |
100.00%
|
100.00%
|
| Primer 3018 | Leptospira biflexa | ACGGTTTGGGTGGCTCAA | TGTCGGTGCAATTCGGGA | 59.40 | 59.26 | 213 | 52 |
100.00%
|
100.00%
|
| Primer 3019 | Leptospira biflexa | TCGCAATGGCTGTGGCAA | TTGTCGGTGCAATTCGGGA | 60.60 | 59.93 | 168 | 52 |
100.00%
|
100.00%
|
| Primer 3020 | Leptospira biflexa | GCGGTCACAGAAACAACGG | TTGGGATTGGCACCTGCA | 59.72 | 59.47 | 164 | 52 |
100.00%
|
100.00%
|