| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 411 | Leptospira biflexa | TCTCTGTGACGGTTTGGGA | TTCACCCGATTGGCAGGAC | 58.17 | 60.00 | 245 | 107 |
100.00%
|
100.00%
|
| Primer 412 | Leptospira biflexa | TGACCCATTCACTTGGTGGA | TGAGTTGGGCAACGACTGG | 58.86 | 60.23 | 174 | 107 |
100.00%
|
100.00%
|
| Primer 413 | Leptospira biflexa | TGACCCATTCACTTGGTGGA | AGTTGGGCAACGACTGGTC | 58.86 | 60.23 | 172 | 107 |
100.00%
|
100.00%
|
| Primer 414 | Leptospira biflexa | CCTGGTTCTTGGCAATTCCC | TTCATCGAGCCCACCATCC | 59.10 | 59.47 | 237 | 107 |
100.00%
|
100.00%
|
| Primer 415 | Leptospira biflexa | ACTTGCCGTGAAGCAGCT | GGCTCACCTACGTTTGCAC | 59.89 | 59.13 | 212 | 106 |
100.00%
|
100.00%
|
| Primer 416 | Leptospira biflexa | ACTTGCCGTGAAGCAGCT | ACCCCAACGCCTTTGATGAT | 59.89 | 59.96 | 155 | 106 |
100.00%
|
100.00%
|
| Primer 417 | Leptospira biflexa | ACTTGCCGTGAAGCAGCT | TCCCAAGTTTCTGGCTCACC | 59.89 | 59.89 | 224 | 106 |
100.00%
|
100.00%
|
| Primer 418 | Leptospira biflexa | ACTTGCCGTGAAGCAGCT | CTGGCTCACCTACGTTTGC | 59.89 | 58.84 | 214 | 106 |
100.00%
|
100.00%
|
| Primer 419 | Leptospira biflexa | TGGTGCCGTGATCACTGT | ACGGTAAGCGGAGGAAGGT | 58.85 | 60.61 | 298 | 106 |
100.00%
|
100.00%
|
| Primer 420 | Leptospira biflexa | TGCTTCGACTGGTGGGTTC | TTTGTCAGGGCTCCGAGTG | 59.93 | 59.63 | 177 | 106 |
100.00%
|
100.00%
|