| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 521 | Leptospira borgpetersenii | ATCGCGATTGCACGGGTA | CCGCAATCCTGTCCGAGAT | 59.50 | 59.56 | 193 | 58 |
100.00%
|
100.00%
|
| Primer 522 | Leptospira borgpetersenii | TGCTCGCTTACGGCACAA | AGTTCGGTCCCACCAGTCT | 59.97 | 60.15 | 291 | 58 |
100.00%
|
100.00%
|
| Primer 523 | Leptospira borgpetersenii | CCGTTTTACTCGAGGGACGG | AATCCCCTGCGCTCATCT | 60.46 | 58.35 | 229 | 58 |
100.00%
|
100.00%
|
| Primer 524 | Leptospira borgpetersenii | AAATCGGTGGGATCGGTGG | ATCGCTGTCGTCCGGAGTA | 59.78 | 60.45 | 191 | 58 |
100.00%
|
100.00%
|
| Primer 525 | Leptospira borgpetersenii | CCCGACACGAGCAAATTGA | CGGTTCCACCTGCAAGTCT | 58.46 | 59.93 | 190 | 58 |
100.00%
|
100.00%
|
| Primer 526 | Leptospira borgpetersenii | TCCCGACACGAGCAAATTG | CGGTTCCACCTGCAAGTCT | 58.46 | 59.93 | 191 | 58 |
100.00%
|
100.00%
|
| Primer 527 | Leptospira borgpetersenii | TTCCCGACACGAGCAAATTG | CGGTTCCACCTGCAAGTCT | 59.13 | 59.93 | 192 | 58 |
100.00%
|
100.00%
|
| Primer 528 | Leptospira borgpetersenii | ACCTGACGGAACCGGATCA | AAACGACCGAGTTCGCCA | 60.61 | 59.58 | 279 | 57 |
100.00%
|
100.00%
|
| Primer 529 | Leptospira borgpetersenii | TGTCAGCCAGGGCAAACT | TCGCCACTTCTCCACCTTG | 59.07 | 59.63 | 182 | 57 |
100.00%
|
100.00%
|
| Primer 530 | Leptospira borgpetersenii | CCGCAAGTAGAACAGGGCA | TGCCTGGTTCCCTTTTCTCC | 60.00 | 59.89 | 288 | 57 |
100.00%
|
100.00%
|