| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Limosilactobacillus fermentum | TGCAACCGCAGCTGTTGA | CGGAACCTTGCTTGCAGGA | 60.52 | 60.60 | 172 | 132 |
100.00%
|
60.00%
|
| Primer 2 | Limosilactobacillus fermentum | TGTACTGTGCCGACCAGAC | TCAAAAGCACCCCTGCAGT | 59.34 | 59.77 | 263 | 130 |
100.00%
|
60.00%
|
| Primer 3 | Limosilactobacillus fermentum | TGCAAAATGACCAAGCGTTTC | AACCGGTGAAGGCGTTACC | 58.53 | 60.30 | 154 | 129 |
100.00%
|
60.00%
|
| Primer 4 | Limosilactobacillus fermentum | AACTCCGTGACGCCAACA | AGCAAGTCTTCGCGACCA | 59.50 | 59.27 | 277 | 124 |
100.00%
|
60.00%
|
| Primer 5 | Limosilactobacillus fermentum | AACGGCAGCCACGTCTTT | AAAGTGCTCCGGAACCACC | 60.20 | 60.23 | 219 | 120 |
100.00%
|
60.00%
|
| Primer 6 | Limosilactobacillus fermentum | AACGGCAGCCACGTCTTT | GCTCCGGAACCACCAGAAT | 60.20 | 59.70 | 214 | 120 |
100.00%
|
60.00%
|
| Primer 7 | Limosilactobacillus fermentum | ACCACACCGTGCAACCAA | GCCGACCGCAAAGCAAAA | 60.04 | 59.97 | 284 | 119 |
100.00%
|
60.00%
|
| Primer 8 | Limosilactobacillus fermentum | TTGGCTTGCTGGTGGTGA | GCCATCATCGTCACCCCAA | 59.40 | 60.08 | 175 | 119 |
100.00%
|
60.00%
|
| Primer 9 | Limosilactobacillus fermentum | ATTGTGCGTGGTCGGGAT | ACTTGAGCCAGCCCTTGAC | 59.33 | 59.93 | 224 | 119 |
100.00%
|
60.00%
|
| Primer 10 | Limosilactobacillus fermentum | TTTGGACGCTTGGGGTGT | CGCGTTGGAAACCGCAAT | 59.40 | 59.75 | 236 | 117 |
100.00%
|
60.00%
|