| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Listeria grayi | TTGGGGTTGTGACAGCAGC | TCGACACGACAACACGCA | 60.83 | 59.90 | 204 | 138 |
100.00%
|
50.00%
|
| Primer 12 | Listeria grayi | CTTTCAGCACGCCTGCAT | GCGGAATGCCATTGTGACC | 58.73 | 59.86 | 166 | 138 |
100.00%
|
50.00%
|
| Primer 13 | Listeria grayi | TCTCGCCATCGTGAAACCC | TGCATAAACAACTGTGCGCA | 60.08 | 59.33 | 169 | 138 |
100.00%
|
50.00%
|
| Primer 14 | Listeria grayi | TCCACAACGGCATGCGAT | CAGCCCTGCATCACGGAAA | 60.05 | 60.68 | 151 | 137 |
100.00%
|
100.00%
|
| Primer 15 | Listeria grayi | GTGGCCAACGTCGCAAAA | TGGGCAGCGACCAATTGT | 59.59 | 59.88 | 199 | 137 |
100.00%
|
50.00%
|
| Primer 16 | Listeria grayi | CGCAGGCGTTCTTGTTCTG | CGCTTGTTGGATACGCTGC | 59.79 | 59.94 | 244 | 135 |
100.00%
|
50.00%
|
| Primer 17 | Listeria grayi | AGGCGACGGCAAAGTTGT | ACCAGTGTAGCCTCCACCA | 60.20 | 60.15 | 154 | 135 |
100.00%
|
50.00%
|
| Primer 18 | Listeria grayi | TTTCAGCACGCCTGCATG | GCGGAATGCCATTGTGACC | 59.04 | 59.86 | 165 | 134 |
100.00%
|
50.00%
|
| Primer 19 | Listeria grayi | CTTTCAGCACGCCTGCATG | GCGGAATGCCATTGTGACC | 60.15 | 59.86 | 166 | 134 |
100.00%
|
50.00%
|
| Primer 20 | Listeria grayi | CAGCACGCCTGCATGTATG | GCGGAATGCCATTGTGACC | 59.65 | 59.86 | 162 | 134 |
100.00%
|
50.00%
|