| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Megasphaera elsdenii | ACGACCAGCAAAGGCCAT | ATGGCGACGATGGTCAAGG | 59.56 | 60.15 | 185 | 118 |
100.00%
|
100.00%
|
| Primer 2 | Megasphaera elsdenii | ACGACCAGCAAAGGCCAT | TTTCGACGAGACGGCTCAG | 59.56 | 59.79 | 211 | 118 |
100.00%
|
100.00%
|
| Primer 3 | Megasphaera elsdenii | ACGCCTTGTTGTCGCCAT | TGTTCTCAGCGCTGTCAGG | 60.28 | 60.01 | 281 | 118 |
100.00%
|
100.00%
|
| Primer 4 | Megasphaera elsdenii | ACGCCTTGTTGTCGCCAT | GGGATGTTCTCAGCGCTGT | 60.28 | 60.08 | 285 | 117 |
100.00%
|
100.00%
|
| Primer 5 | Megasphaera elsdenii | GGGATGTGAATTCGGCAGC | TGACGTCGAACTGCGTGT | 59.27 | 59.59 | 236 | 110 |
100.00%
|
100.00%
|
| Primer 6 | Megasphaera elsdenii | ATTCCTGGCTGGCGAAGTC | TCAGTTCGCCTGCGTTGT | 60.08 | 59.89 | 296 | 110 |
100.00%
|
100.00%
|
| Primer 7 | Megasphaera elsdenii | ACGCCTTGTTGTCGCCAT | GTTCTCAGCGCTGTCAGGA | 60.28 | 59.71 | 280 | 109 |
100.00%
|
100.00%
|
| Primer 8 | Megasphaera elsdenii | TGCAAGGTGACGCTGTGT | TGGCCTCAGCATTACGCA | 59.81 | 59.33 | 163 | 107 |
100.00%
|
100.00%
|
| Primer 9 | Megasphaera elsdenii | TCGTCTCCCTCGGTTCCAT | TCAGTTCCTTGCCGTGCA | 60.00 | 59.49 | 162 | 107 |
100.00%
|
100.00%
|
| Primer 10 | Megasphaera elsdenii | GGCCTGGGCATCATCTTGT | TCAGTTCCTTGCCGTGCA | 60.08 | 59.49 | 247 | 107 |
100.00%
|
100.00%
|