| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Mesomycoplasma hyopneumoniae | TGGACCACCAGGAACAGGA | TCTCCCCATCAACGCCAATC | 60.07 | 60.11 | 251 | 140 |
100.00%
|
100.00%
|
| Primer 2 | Mesomycoplasma hyopneumoniae | TGGACCACCAGGAACAGGA | TTCTCTCCCCATCAACGCC | 60.07 | 59.40 | 254 | 138 |
100.00%
|
100.00%
|
| Primer 3 | Mesomycoplasma hyopneumoniae | TGGACCACCAGGAACAGGA | TTTCTCTCCCCATCAACGCC | 60.07 | 60.04 | 255 | 138 |
100.00%
|
100.00%
|
| Primer 4 | Mesomycoplasma hyopneumoniae | AGCGCAATTGTAGCTGCA | TCTTGTCGGATCAGACGCA | 58.01 | 58.74 | 164 | 138 |
100.00%
|
100.00%
|
| Primer 5 | Mesomycoplasma hyopneumoniae | TGGACCACCAGGAACAGGA | TCTCTCCCCATCAACGCCA | 60.07 | 60.61 | 253 | 135 |
100.00%
|
100.00%
|
| Primer 6 | Mesomycoplasma hyopneumoniae | TGAGATGGCGATGCTCTTGA | CGAATCAAGTCATTCCTGCGG | 59.17 | 59.67 | 204 | 130 |
100.00%
|
100.00%
|
| Primer 7 | Mesomycoplasma hyopneumoniae | TGGACCACCAGGAACAGGA | CTCTCCCCATCAACGCCAA | 60.07 | 59.70 | 252 | 130 |
100.00%
|
100.00%
|
| Primer 8 | Mesomycoplasma hyopneumoniae | TGGACCACCAGGAACAGGA | TCTCCCCATCAACGCCAA | 60.07 | 58.51 | 251 | 130 |
100.00%
|
100.00%
|
| Primer 9 | Mesomycoplasma hyopneumoniae | TGGACCACCAGGAACAGGA | TCTCCCCATCAACGCCAAT | 60.07 | 59.00 | 251 | 128 |
100.00%
|
100.00%
|
| Primer 10 | Mesomycoplasma hyopneumoniae | TGGACCACCAGGAACAGGA | CTCTCCCCATCAACGCCAAT | 60.07 | 60.11 | 252 | 128 |
100.00%
|
100.00%
|