| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Methanobrevibacter smithii | GCGCAGGCTATTGTTGAGG | ACCAGACTCTTCACCACCAG | 59.28 | 58.95 | 186 | 181 |
100.00%
|
50.00%
|
| Primer 2 | Methanobrevibacter smithii | GCGCAGGCTATTGTTGAGG | ACTCTTCACCACCAGTTCCAG | 59.28 | 59.58 | 181 | 181 |
100.00%
|
50.00%
|
| Primer 3 | Methanobrevibacter smithii | GCGCAGGCTATTGTTGAGG | AACCAGACTCTTCACCACCAG | 59.28 | 59.58 | 187 | 181 |
100.00%
|
50.00%
|
| Primer 4 | Methanobrevibacter smithii | GCACTTCCTGTGGCATTGC | CTACCCCTGTTGCCATTGC | 60.08 | 58.81 | 250 | 157 |
100.00%
|
100.00%
|
| Primer 5 | Methanobrevibacter smithii | GCTGAAGAACTGCACGAAGC | GCAGTCGCGAACATGCAT | 60.11 | 59.21 | 195 | 149 |
100.00%
|
50.00%
|
| Primer 6 | Methanobrevibacter smithii | ATGATGAAACCGGACGCAG | TCCGAGGTCTGCTTCTAAAGG | 58.23 | 59.17 | 279 | 148 |
100.00%
|
100.00%
|
| Primer 7 | Methanobrevibacter smithii | ATGGGCAAAGGTGGAGGTC | TGAGGAACACCTGCACCTG | 59.62 | 59.55 | 188 | 147 |
100.00%
|
100.00%
|
| Primer 8 | Methanobrevibacter smithii | GATAGGGATGTGGCCAGAGC | TGGAAGCCATAGCACCTGC | 59.96 | 60.08 | 151 | 146 |
100.00%
|
50.00%
|
| Primer 9 | Methanobrevibacter smithii | GCGCAGGCTATTGTTGAGG | CCAGACTCTTCACCACCAGT | 59.28 | 58.95 | 185 | 143 |
100.00%
|
50.00%
|
| Primer 10 | Methanobrevibacter smithii | CGCTGAAGAACTGCACGAAG | GCAGTCGCGAACATGCAT | 59.84 | 59.21 | 196 | 140 |
100.00%
|
50.00%
|