| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Methanosphaera cuniculi | GCATGGGCACAAGTTGATGT | TCAGCAACACCCTGTGCA | 59.40 | 59.41 | 155 | 138 |
100.00%
|
100.00%
|
| Primer 2 | Methanosphaera cuniculi | GCATGGGCACAAGTTGATGT | TCAGCAACACCCTGTGCAT | 59.40 | 59.85 | 155 | 137 |
100.00%
|
100.00%
|
| Primer 3 | Methanosphaera cuniculi | GCATGGGCACAAGTTGATGT | CAGCAACACCCTGTGCAT | 59.40 | 58.23 | 154 | 137 |
100.00%
|
100.00%
|
| Primer 4 | Methanosphaera cuniculi | GCATGGGCACAAGTTGATGT | TTCAGCAACACCCTGTGC | 59.40 | 58.16 | 156 | 137 |
100.00%
|
100.00%
|
| Primer 5 | Methanosphaera cuniculi | GCAGCAGCAGTAGCATCAC | ACACGGCTTAGTGGTGGTTC | 59.28 | 60.25 | 176 | 136 |
100.00%
|
100.00%
|
| Primer 6 | Methanosphaera cuniculi | GCAGCAGCAGTAGCATCAC | CACGGCTTAGTGGTGGTTC | 59.28 | 58.46 | 175 | 136 |
100.00%
|
100.00%
|
| Primer 7 | Methanosphaera cuniculi | ACGTAACCACCCCAAGCAG | CCTCCATCATCTGCAAGTGC | 59.93 | 58.98 | 181 | 135 |
100.00%
|
100.00%
|
| Primer 8 | Methanosphaera cuniculi | AACCACCCCAAGCAGTAGC | CCTCCATCATCTGCAAGTGC | 59.93 | 58.98 | 177 | 135 |
100.00%
|
100.00%
|
| Primer 9 | Methanosphaera cuniculi | AGACGTAACCACCCCAAGC | CCTCCATCATCTGCAAGTGC | 59.63 | 58.98 | 183 | 135 |
100.00%
|
100.00%
|
| Primer 10 | Methanosphaera cuniculi | GGAGATGATGGACTAGGACCA | TCCCCAGGTGTTGTGTCAAA | 58.04 | 59.37 | 197 | 135 |
100.00%
|
100.00%
|