| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 101 | Methanosphaera cuniculi | TTAACGCACCTGACTGGGC | TCCACGGTCTTTGTTGCCA | 60.30 | 59.78 | 176 | 113 |
100.00%
|
100.00%
|
| Primer 102 | Methanosphaera cuniculi | AGTAACGGGTGGAGCAGGA | CCTCACCATTCCAACGACCA | 60.23 | 59.96 | 221 | 113 |
100.00%
|
100.00%
|
| Primer 103 | Methanosphaera cuniculi | GTCCTGCACTTGGAGCTGT | TGCTCCTTCTTGGCGATCTAC | 59.93 | 59.86 | 260 | 113 |
100.00%
|
100.00%
|
| Primer 104 | Methanosphaera cuniculi | CTGTAAGTGCATGGGCTCCA | TCTCCACGTTCTGGTGTGT | 60.04 | 58.49 | 169 | 113 |
100.00%
|
100.00%
|
| Primer 105 | Methanosphaera cuniculi | TGTAAGTGCATGGGCTCCA | TCTCCACGTTCTGGTGTGT | 58.93 | 58.49 | 168 | 113 |
100.00%
|
100.00%
|
| Primer 106 | Methanosphaera cuniculi | AGGACCACATGCATGTACTGG | TGATGCCCTCAGCTTCCAC | 60.07 | 59.70 | 188 | 113 |
100.00%
|
100.00%
|
| Primer 107 | Methanosphaera cuniculi | AGGCGACGATGCAGATGT | GGGTTTGTCCATCTGCGATG | 59.10 | 59.27 | 191 | 112 |
100.00%
|
100.00%
|
| Primer 108 | Methanosphaera cuniculi | TGTAAGACGAGACTGGGCAC | GCTGCTATTACGTGTGGTGC | 59.40 | 59.63 | 219 | 112 |
100.00%
|
100.00%
|
| Primer 109 | Methanosphaera cuniculi | TTGTAAGACGAGACTGGGCAC | GCTGCTATTACGTGTGGTGC | 60.00 | 59.63 | 220 | 112 |
100.00%
|
100.00%
|
| Primer 110 | Methanosphaera cuniculi | GGAACATGGCGTGCAATGG | CAGCAAGTGGTGACCATGC | 60.15 | 59.42 | 220 | 112 |
100.00%
|
100.00%
|