| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 271 | Methanosphaera cuniculi | CGCAGATGCTGATGGGCTA | TCACCATGTCCTGTTCCTCC | 59.93 | 59.02 | 270 | 89 |
100.00%
|
100.00%
|
| Primer 272 | Methanosphaera cuniculi | CGCAGATGCTGATGGGCTA | CTCCATAGTGTCCTGTTGGCA | 59.93 | 59.72 | 254 | 89 |
100.00%
|
100.00%
|
| Primer 273 | Methanosphaera cuniculi | CGCAGATGCTGATGGGCTA | CACCATGTCCTGTTCCTCCAT | 59.93 | 59.72 | 269 | 89 |
100.00%
|
100.00%
|
| Primer 274 | Methanosphaera cuniculi | AGCCCAGGAAAAGCTGGAC | TCCTGGTGTGTTTGGTCCT | 59.92 | 58.37 | 261 | 89 |
100.00%
|
100.00%
|
| Primer 275 | Methanosphaera cuniculi | GCCCAGGAAAAGCTGGACA | TCCTGGTGTGTTTGGTCCT | 60.23 | 58.37 | 260 | 89 |
100.00%
|
100.00%
|
| Primer 276 | Methanosphaera cuniculi | GCAGGAACAAGCCCAGGAA | TCCTGGTGTGTTTGGTCCT | 60.23 | 58.37 | 270 | 89 |
100.00%
|
100.00%
|
| Primer 277 | Methanosphaera cuniculi | AGCCCAGGAAAAGCTGGA | TCCTGGTGTGTTTGGTCCT | 58.41 | 58.37 | 261 | 89 |
100.00%
|
100.00%
|
| Primer 278 | Methanosphaera cuniculi | TCACGAGAACACACCCAAGT | TGGGCAGAGCACTTCTCCT | 59.17 | 60.54 | 228 | 89 |
100.00%
|
100.00%
|
| Primer 279 | Methanosphaera cuniculi | TCACGAGAACACACCCAAGT | TGGGCAGAGCACTTCTCCTA | 59.17 | 60.25 | 228 | 89 |
100.00%
|
100.00%
|
| Primer 280 | Methanosphaera cuniculi | TCGTTGGAACAGGCGAAGT | GTGTTGTTTGTTGCAGCTACG | 59.56 | 58.89 | 199 | 88 |
100.00%
|
100.00%
|