| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Methanosphaera cuniculi | GGAGATGATGGACTAGGACCA | TCCCCAGGTGTTGTGTCAA | 58.04 | 58.69 | 197 | 130 |
100.00%
|
100.00%
|
| Primer 22 | Methanosphaera cuniculi | GGAGATGATGGACTAGGACCA | TTCCCCAGGTGTTGTGTCAA | 58.04 | 59.37 | 198 | 130 |
100.00%
|
100.00%
|
| Primer 23 | Methanosphaera cuniculi | GGAGATGATGGACTAGGACCA | TTTCCCCAGGTGTTGTGTCAA | 58.04 | 59.99 | 199 | 130 |
100.00%
|
100.00%
|
| Primer 24 | Methanosphaera cuniculi | TGTCTTCCACGTGTTGGTGT | CACACCAGCTTGAACCGGA | 59.75 | 60.23 | 177 | 129 |
100.00%
|
100.00%
|
| Primer 25 | Methanosphaera cuniculi | TGTCTTCCACGTGTTGGTGT | ACACCAGCTTGAACCGGA | 59.75 | 58.76 | 176 | 129 |
100.00%
|
100.00%
|
| Primer 26 | Methanosphaera cuniculi | CCATGTCCTCCAACAGTGCT | ACGTCTTGCAGCCCAACA | 59.96 | 59.81 | 282 | 128 |
100.00%
|
100.00%
|
| Primer 27 | Methanosphaera cuniculi | CACCAATGCACAACACAGCA | ACAGCTCCTATTTTCTGGGCT | 59.90 | 59.08 | 205 | 128 |
100.00%
|
100.00%
|
| Primer 28 | Methanosphaera cuniculi | CACCACCAATGCACAACACA | ACAGCTCCTATTTTCTGGGCT | 59.54 | 59.08 | 208 | 128 |
100.00%
|
100.00%
|
| Primer 29 | Methanosphaera cuniculi | ACCAATGCACAACACAGCA | ACAGCTCCTATTTTCTGGGCT | 58.50 | 59.08 | 204 | 128 |
100.00%
|
100.00%
|
| Primer 30 | Methanosphaera cuniculi | CCACCAATGCACAACACAGC | ACAGCTCCTATTTTCTGGGCT | 60.60 | 59.08 | 206 | 128 |
100.00%
|
100.00%
|