| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 301 | Methanosphaera cuniculi | AGTAACGGGTGGAGCAGGA | TCCTCACCATTCCAACGACC | 60.23 | 59.68 | 222 | 84 |
100.00%
|
100.00%
|
| Primer 302 | Methanosphaera cuniculi | CGCACCAAAAGGACCAGGA | AACAACTCCAGCACGGGT | 60.23 | 59.08 | 157 | 84 |
100.00%
|
100.00%
|
| Primer 303 | Methanosphaera cuniculi | TCGCACCAAAAGGACCAGG | AACAACTCCAGCACGGGT | 60.23 | 59.08 | 158 | 84 |
100.00%
|
100.00%
|
| Primer 304 | Methanosphaera cuniculi | CGCACCAAAAGGACCAGGA | CAACAACTCCAGCACGGGT | 60.23 | 60.53 | 158 | 84 |
100.00%
|
100.00%
|
| Primer 305 | Methanosphaera cuniculi | TCGCACCAAAAGGACCAGG | CAACAACTCCAGCACGGGT | 60.23 | 60.53 | 159 | 84 |
100.00%
|
100.00%
|
| Primer 306 | Methanosphaera cuniculi | AGCAGCACAACTAGCACCA | TTGCACGGCTTACTACTGCT | 59.55 | 59.68 | 152 | 84 |
100.00%
|
100.00%
|
| Primer 307 | Methanosphaera cuniculi | AGCAGCACAACTAGCACCAA | TGCACGGCTTACTACTGCT | 60.18 | 59.03 | 151 | 84 |
100.00%
|
100.00%
|
| Primer 308 | Methanosphaera cuniculi | AAGCAGCACAACTAGCACCA | TGCACGGCTTACTACTGCT | 60.18 | 59.03 | 152 | 84 |
100.00%
|
100.00%
|
| Primer 309 | Methanosphaera cuniculi | AAGTTGCAGCAACCCGAAAA | AGCTCCAAAGAGTGCTGCT | 59.18 | 59.24 | 251 | 84 |
100.00%
|
100.00%
|
| Primer 310 | Methanosphaera cuniculi | AAAGTTGCAGCAACCCGAAA | AGCTCCAAAGAGTGCTGCT | 59.18 | 59.24 | 252 | 84 |
100.00%
|
100.00%
|