| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 501 | Methanosphaera cuniculi | TTCACAAACCTCTCTACGTGC | TGGGCTGTAGCTTGATTGCA | 58.25 | 59.96 | 168 | 71 |
100.00%
|
100.00%
|
| Primer 502 | Methanosphaera cuniculi | AGATCCATGGTGGGCAGGA | TCACCAACTTCTGCTCCACA | 60.31 | 59.16 | 282 | 70 |
100.00%
|
100.00%
|
| Primer 503 | Methanosphaera cuniculi | AGGACCACATGCATGTACTGG | ATGCCCTCAGCTTCCACA | 60.07 | 58.50 | 186 | 70 |
100.00%
|
100.00%
|
| Primer 504 | Methanosphaera cuniculi | AGGTTACGGAGCTACTAGGCT | TGGTGCCCAGTCTCGTCTT | 59.79 | 60.84 | 173 | 69 |
100.00%
|
100.00%
|
| Primer 505 | Methanosphaera cuniculi | TTTACTGCTTTGTCCTGGCTT | AGAACAATGCCCAACCAAAGA | 58.06 | 58.32 | 179 | 69 |
100.00%
|
100.00%
|
| Primer 506 | Methanosphaera cuniculi | TGGCGTGCAATGGAAGAGT | AGCAAGTGGTGACCATGCTT | 59.93 | 60.18 | 213 | 69 |
100.00%
|
100.00%
|
| Primer 507 | Methanosphaera cuniculi | ACACGTGCAGGTGGAAAGA | TGCTGTTGGTTCTCCCCA | 59.48 | 58.33 | 207 | 69 |
100.00%
|
100.00%
|
| Primer 508 | Methanosphaera cuniculi | ACAAACCTCTCTACGTGCTGA | TGGACTTTGGGCTGTAGCTT | 59.04 | 59.23 | 172 | 69 |
100.00%
|
100.00%
|
| Primer 509 | Methanosphaera cuniculi | TCACAAACCTCTCTACGTGCT | TGGACTTTGGGCTGTAGCTT | 59.04 | 59.23 | 174 | 69 |
100.00%
|
100.00%
|
| Primer 510 | Methanosphaera cuniculi | CACAAACCTCTCTACGTGCTG | TGGACTTTGGGCTGTAGCTT | 58.94 | 59.23 | 173 | 69 |
100.00%
|
100.00%
|