| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 621 | Methanosphaera cuniculi | AAACCCAATGGCTACCGGT | TTGAACGAGGTCTGCTCCT | 59.23 | 58.25 | 229 | 55 |
100.00%
|
100.00%
|
| Primer 622 | Methanosphaera cuniculi | AGATCCATGGTGGGCAGGA | CAGGATGTGTGTGTCCTCCT | 60.31 | 59.02 | 194 | 55 |
100.00%
|
100.00%
|
| Primer 623 | Methanosphaera cuniculi | TGGACAATGGACTAGGGCAA | ACCTGGTGGTGTTCCCGTT | 58.63 | 61.38 | 232 | 55 |
100.00%
|
100.00%
|
| Primer 624 | Methanosphaera cuniculi | GATGGACAATGGACTAGGGCA | ACCTGGTGGTGTTCCCGTT | 59.51 | 61.38 | 234 | 55 |
100.00%
|
100.00%
|
| Primer 625 | Methanosphaera cuniculi | TGCCCATACTGTCCTATGGC | TTGTGCTGATGGTGCTCCT | 59.24 | 59.24 | 186 | 55 |
100.00%
|
100.00%
|
| Primer 626 | Methanosphaera cuniculi | GCCCATACTGTCCTATGGCAG | TGTGCTGATGGTGCTCCT | 60.27 | 58.51 | 184 | 55 |
100.00%
|
100.00%
|
| Primer 627 | Methanosphaera cuniculi | ATGCCCATACTGTCCTATGGC | TGTGCTGATGGTGCTCCT | 59.65 | 58.51 | 186 | 55 |
100.00%
|
100.00%
|
| Primer 628 | Methanosphaera cuniculi | CTGTAAGTGCATGGGCTCCA | TCTCCACGTTCTGGTGTGTT | 60.04 | 59.17 | 169 | 55 |
100.00%
|
100.00%
|
| Primer 629 | Methanosphaera cuniculi | TGTAAGTGCATGGGCTCCA | TCTCCACGTTCTGGTGTGTT | 58.93 | 59.17 | 168 | 55 |
100.00%
|
100.00%
|
| Primer 630 | Methanosphaera cuniculi | ACAATACGTGAAGCTCCCCT | AACACAGCTTGCTGTTGGT | 58.72 | 58.11 | 180 | 55 |
100.00%
|
100.00%
|