| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Methanosphaera stadtmanae | TCTTGCAGGAGATCAGCCAAC | GGGCATACCTAAACCTTCAGC | 60.34 | 58.70 | 151 | 151 |
100.00%
|
100.00%
|
| Primer 2 | Methanosphaera stadtmanae | GGGATCAGTTGCAAAGGGC | TGTACCAATTCCTCCTGGTGT | 59.11 | 58.66 | 193 | 143 |
100.00%
|
100.00%
|
| Primer 3 | Methanosphaera stadtmanae | TGGGATCAGTTGCAAAGGGC | TGTACCAATTCCTCCTGGTGT | 60.90 | 58.66 | 194 | 143 |
100.00%
|
100.00%
|
| Primer 4 | Methanosphaera stadtmanae | ATGGGATCAGTTGCAAAGGGC | TGTACCAATTCCTCCTGGTGT | 61.24 | 58.66 | 195 | 143 |
100.00%
|
100.00%
|
| Primer 5 | Methanosphaera stadtmanae | GCGTAAGGCATGTTATGAGGA | TCACCACCACAGGATAGGGC | 58.17 | 61.27 | 177 | 140 |
100.00%
|
100.00%
|
| Primer 6 | Methanosphaera stadtmanae | GCGTAAGGCATGTTATGAGGA | CCACCACAGGATAGGGCTAG | 58.17 | 58.95 | 174 | 138 |
100.00%
|
100.00%
|
| Primer 7 | Methanosphaera stadtmanae | GCGTAAGGCATGTTATGAGGA | ACCACCACAGGATAGGGCTAG | 58.17 | 60.69 | 175 | 138 |
100.00%
|
100.00%
|
| Primer 8 | Methanosphaera stadtmanae | ACCTGCAGAAATTGTAGCACA | TCTTGGCCTTCACCAGCA | 58.14 | 58.75 | 153 | 137 |
100.00%
|
100.00%
|
| Primer 9 | Methanosphaera stadtmanae | ACCTGCAGAAATTGTAGCACA | TTCTTGGCCTTCACCAGCA | 58.14 | 59.46 | 154 | 137 |
100.00%
|
100.00%
|
| Primer 10 | Methanosphaera stadtmanae | ACCTGCAGAAATTGTAGCACA | TCTTGGCCTTCACCAGCAT | 58.14 | 59.23 | 153 | 137 |
100.00%
|
100.00%
|