| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Methanosphaera stadtmanae | ACAGAACCTGCTGGATGATCT | CCCCATGTTACAACTGCCAC | 58.81 | 59.11 | 228 | 127 |
100.00%
|
100.00%
|
| Primer 42 | Methanosphaera stadtmanae | ACAGAACCTGCTGGATGATCT | GACCCCATGTTACAACTGCC | 58.81 | 58.83 | 230 | 127 |
100.00%
|
100.00%
|
| Primer 43 | Methanosphaera stadtmanae | ACAGAACCTGCTGGATGATCT | TCCCTGACCCCATGTTACAAC | 58.81 | 59.65 | 235 | 127 |
100.00%
|
100.00%
|
| Primer 44 | Methanosphaera stadtmanae | ACAGAACCTGCTGGATGATCT | CCCTGACCCCATGTTACAACT | 58.81 | 59.65 | 234 | 127 |
100.00%
|
100.00%
|
| Primer 45 | Methanosphaera stadtmanae | ACAGAACCTGCTGGATGATCT | GACCCCATGTTACAACTGCCA | 58.81 | 60.55 | 230 | 127 |
100.00%
|
100.00%
|
| Primer 46 | Methanosphaera stadtmanae | ACAGAACCTGCTGGATGATCT | TGACCCCATGTTACAACTGCC | 58.81 | 60.55 | 231 | 127 |
100.00%
|
100.00%
|
| Primer 47 | Methanosphaera stadtmanae | ACAGAACCTGCTGGATGATCT | TCCCTGACCCCATGTTACAA | 58.81 | 58.25 | 235 | 127 |
100.00%
|
100.00%
|
| Primer 48 | Methanosphaera stadtmanae | ACAGAACCTGCTGGATGATCT | TTCCCTGACCCCATGTTACA | 58.81 | 58.25 | 236 | 127 |
100.00%
|
100.00%
|
| Primer 49 | Methanosphaera stadtmanae | GGCTGTAAAAGGTCATGGAGC | ACATCGTATGTTCCATCGGCA | 59.25 | 59.86 | 207 | 127 |
100.00%
|
100.00%
|
| Primer 50 | Methanosphaera stadtmanae | GGCTGTAAAAGGTCATGGAGC | CGTATGTTCCATCGGCAACAT | 59.25 | 59.06 | 203 | 127 |
100.00%
|
100.00%
|