| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 231 | Methylocella silvestris | GCTTCACGTCGGACCTGTT | TCCGCTTGCCATTGAGCA | 60.30 | 59.97 | 297 | 100 |
100.00%
|
100.00%
|
| Primer 232 | Methylocella silvestris | ATTTTCGCGAACGCCACG | AATCCTGCCAAGGTCGCA | 59.83 | 59.24 | 203 | 99 |
100.00%
|
100.00%
|
| Primer 233 | Methylocella silvestris | ATTTTCGCGAACGCCACG | ATCCTGCCAAGGTCGCAAA | 59.83 | 59.93 | 202 | 99 |
100.00%
|
100.00%
|
| Primer 234 | Methylocella silvestris | ATTTTCGCGAACGCCACG | AAATCCTGCCAAGGTCGCA | 59.83 | 59.93 | 204 | 99 |
100.00%
|
100.00%
|
| Primer 235 | Methylocella silvestris | ATTTTCGCGAACGCCACG | AGGCCTTCCGGACGATAGT | 59.83 | 60.08 | 239 | 99 |
100.00%
|
100.00%
|
| Primer 236 | Methylocella silvestris | ACATCTTCGCTGCGCTGT | TTTCAGCGACGCAAAGGC | 60.05 | 59.36 | 222 | 99 |
100.00%
|
100.00%
|
| Primer 237 | Methylocella silvestris | ACATCTTCGCTGCGCTGT | TTTTCAGCGACGCAAAGGC | 60.05 | 60.01 | 223 | 99 |
100.00%
|
100.00%
|
| Primer 238 | Methylocella silvestris | ACAAGACGGTCGCCATGA | TTCCGACTCGAACGCGAT | 58.94 | 59.13 | 261 | 99 |
100.00%
|
100.00%
|
| Primer 239 | Methylocella silvestris | TCGATTGGCTCTGCGTCA | TTGGTGTTGGCGTCGACA | 59.03 | 59.81 | 175 | 99 |
100.00%
|
100.00%
|
| Primer 240 | Methylocella silvestris | GGCTCCCTCGAAATACTGCA | TGCGCTCGCCTTCTTTCA | 59.82 | 59.97 | 295 | 99 |
100.00%
|
100.00%
|