| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Methylovorus glucosotrophus | GGAGTATTTGGAGCAGGCCA | TGCACGCCACTCATGCA | 59.74 | 59.93 | 169 | 135 |
100.00%
|
100.00%
|
| Primer 42 | Methylovorus glucosotrophus | CCAGAGCGAACAAGTGCCA | ACGGCTTCTTTGGTCGCA | 60.60 | 59.89 | 249 | 135 |
100.00%
|
100.00%
|
| Primer 43 | Methylovorus glucosotrophus | ATTTGCTTGGCTGCTGGC | TTGGTCTTGGTGCGCTGA | 59.34 | 59.49 | 218 | 132 |
100.00%
|
100.00%
|
| Primer 44 | Methylovorus glucosotrophus | AACGAACTGTGGCTGGCA | TTGCAAGCGCTGGGTCA | 59.81 | 59.84 | 255 | 132 |
100.00%
|
100.00%
|
| Primer 45 | Methylovorus glucosotrophus | TGGAATCCAACCCAAGAGAGC | TGCAGAGGCCAAATCCAGG | 60.00 | 60.00 | 182 | 132 |
100.00%
|
100.00%
|
| Primer 46 | Methylovorus glucosotrophus | TCTGCTGCGTGCGATTGA | TTGCGCACCAGCTCTTCA | 60.05 | 59.89 | 216 | 132 |
100.00%
|
100.00%
|
| Primer 47 | Methylovorus glucosotrophus | TGCCTTGCTGTTTGGCCA | TCCAGCCAGGTACACCCAA | 60.44 | 60.46 | 196 | 130 |
100.00%
|
100.00%
|
| Primer 48 | Methylovorus glucosotrophus | TGGATGGCAATCTGCTCGG | TGATGTCGCCATGCTGCA | 60.15 | 60.05 | 225 | 130 |
100.00%
|
100.00%
|
| Primer 49 | Methylovorus glucosotrophus | AGGCGTGGCATTGCTGAT | AGAGCCAATCACGCATCCC | 60.04 | 60.15 | 151 | 129 |
100.00%
|
100.00%
|
| Primer 50 | Methylovorus glucosotrophus | ATTTGCCAGTGCGCTTGC | TGTACCGGTTTTGGCTCCC | 60.05 | 59.93 | 286 | 129 |
100.00%
|
100.00%
|