| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Mycolicibacterium smegmatis | ACGGATTTCCACCTCGGTG | TTTTGATGCCGTCGGTGC | 59.70 | 59.05 | 217 | 101 |
100.00%
|
100.00%
|
| Primer 22 | Mycolicibacterium smegmatis | TGTTCCACCAGCAGCTCAC | TGCAACGCGAGCAGTTTG | 60.23 | 59.67 | 199 | 100 |
100.00%
|
100.00%
|
| Primer 23 | Mycolicibacterium smegmatis | TGTGTTCCACCAGCAGCTC | TGCAACGCGAGCAGTTTG | 60.23 | 59.67 | 201 | 100 |
100.00%
|
100.00%
|
| Primer 24 | Mycolicibacterium smegmatis | GCGAGGAATGCACCAAAGC | AGCGTCTTGATGTCGGGTG | 60.15 | 60.08 | 208 | 100 |
100.00%
|
100.00%
|
| Primer 25 | Mycolicibacterium smegmatis | TACATCGGCATGCGTGAGG | CGCATGCGATGTAGCGTTC | 60.23 | 60.08 | 175 | 100 |
100.00%
|
100.00%
|
| Primer 26 | Mycolicibacterium smegmatis | ACGGATTTCCACCTCGGTG | GGTTTTGATGCCGTCGGTG | 59.70 | 59.79 | 219 | 100 |
100.00%
|
100.00%
|
| Primer 27 | Mycolicibacterium smegmatis | TCGACAAGGCCAAACGCA | GATGCCGAGCAGTTTGAGC | 60.20 | 59.57 | 206 | 98 |
100.00%
|
100.00%
|
| Primer 28 | Mycolicibacterium smegmatis | AGCGGACGGTGAAGATGTG | ATTCCAGGAACGCACGCA | 60.08 | 59.97 | 158 | 98 |
100.00%
|
100.00%
|
| Primer 29 | Mycolicibacterium smegmatis | ATGGCGTGACGACCTTCTG | ATTCCAGGAACGCACGCA | 60.08 | 59.97 | 188 | 98 |
100.00%
|
100.00%
|
| Primer 30 | Mycolicibacterium smegmatis | GAGCGGACGGTGAAGATGT | ATTCCAGGAACGCACGCA | 59.78 | 59.97 | 159 | 98 |
100.00%
|
100.00%
|