| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Mycoplasmopsis pulmonis | TGGTTTGCATGCAAGACCTG | GCTTTGATGGCGTTGATTGC | 59.32 | 59.01 | 201 | 148 |
100.00%
|
100.00%
|
| Primer 2 | Mycoplasmopsis pulmonis | TGAGCAGCTGAATCTGGTGT | GCGCAACCTGTTTCAGAGC | 59.31 | 60.08 | 158 | 138 |
100.00%
|
100.00%
|
| Primer 3 | Mycoplasmopsis pulmonis | ATGAGCAGCTGAATCTGGTGT | GCGCAACCTGTTTCAGAGC | 59.72 | 60.08 | 159 | 138 |
100.00%
|
100.00%
|
| Primer 4 | Mycoplasmopsis pulmonis | AGGCCAAAGCTCTAGGAGAG | CTCGCTTTGCAGTGCAGT | 58.51 | 58.66 | 281 | 133 |
100.00%
|
100.00%
|
| Primer 5 | Mycoplasmopsis pulmonis | AGCACTAAAGGCCAAAGCTC | CTCGCTTTGCAGTGCAGT | 58.46 | 58.66 | 289 | 133 |
100.00%
|
100.00%
|
| Primer 6 | Mycoplasmopsis pulmonis | AAGGCCAAAGCTCTAGGAGAG | CTCGCTTTGCAGTGCAGT | 59.16 | 58.66 | 282 | 133 |
100.00%
|
100.00%
|
| Primer 7 | Mycoplasmopsis pulmonis | GAGACGGAAAACACGACGAAG | GCGCAACCTGTTTCAGAGC | 59.55 | 60.08 | 187 | 130 |
100.00%
|
100.00%
|
| Primer 8 | Mycoplasmopsis pulmonis | TGCAAGTCAAGCTGCACAT | GGCTTTTGGAAATGAAAGGCC | 58.28 | 58.57 | 202 | 128 |
100.00%
|
100.00%
|
| Primer 9 | Mycoplasmopsis pulmonis | ATGCAAGTCAAGCTGCACAT | GGCTTTTGGAAATGAAAGGCC | 58.75 | 58.57 | 203 | 128 |
100.00%
|
100.00%
|
| Primer 10 | Mycoplasmopsis pulmonis | TATGCAAGTCAAGCTGCACAT | GGCTTTTGGAAATGAAAGGCC | 58.56 | 58.57 | 204 | 128 |
100.00%
|
100.00%
|