| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 211 | Peribacillus simplex | TGTCGGCAAAGCGACAGT | AACATCGCTGCGCAGGA | 59.89 | 59.68 | 198 | 100 |
100.00%
|
100.00%
|
| Primer 212 | Peribacillus simplex | TGGGATGGAGACCTTTCGGA | ACGGCATGCAGTTCTTTGTG | 60.25 | 59.69 | 257 | 100 |
100.00%
|
100.00%
|
| Primer 213 | Peribacillus simplex | AGGGGTGAAGCTGCTTACG | ATGCCAGCCATGCAGCAA | 59.70 | 60.68 | 209 | 100 |
100.00%
|
100.00%
|
| Primer 214 | Peribacillus simplex | ACGTTCGAAGCGACGGAT | GCTCTTGAAGCCCTTCGGA | 59.44 | 59.70 | 166 | 100 |
100.00%
|
100.00%
|
| Primer 215 | Peribacillus simplex | AAGCTGGGCTCAATCGAGG | TTGCAGCGATTCATGGCAA | 59.78 | 58.73 | 153 | 100 |
100.00%
|
100.00%
|
| Primer 216 | Peribacillus simplex | GCAGCATTGTACCGAGTGG | AGCGATGACTAATGCTTCCCC | 58.91 | 60.20 | 216 | 100 |
100.00%
|
100.00%
|
| Primer 217 | Peribacillus simplex | GCAGCATTGTACCGAGTGG | GCGATGACTAATGCTTCCCC | 58.91 | 58.77 | 215 | 100 |
100.00%
|
100.00%
|
| Primer 218 | Peribacillus simplex | TGGAAGGAAACCGCACCA | CATGGGTGTCCTCGAGCAA | 59.08 | 59.70 | 211 | 100 |
100.00%
|
100.00%
|
| Primer 219 | Peribacillus simplex | ACAATGGCTGCCGTTTGC | TCGGTGCAAACCAGTGGA | 59.66 | 59.09 | 157 | 100 |
100.00%
|
100.00%
|
| Primer 220 | Peribacillus simplex | ACAATGGCTGCCGTTTGC | TTCGGTGCAAACCAGTGGA | 59.66 | 59.78 | 158 | 100 |
100.00%
|
100.00%
|