| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Prevotella ruminicola | ACACCGTTAACCGCTCTACC | TCAACCTCAACACCGCTGG | 59.76 | 60.23 | 151 | 171 |
100.00%
|
100.00%
|
| Primer 2 | Prevotella ruminicola | ATCGACGAGAACAGCCCTG | AGCAGTGCCTTGTCGCAT | 59.49 | 59.97 | 287 | 138 |
100.00%
|
100.00%
|
| Primer 3 | Prevotella ruminicola | TGGATGCCAATCACGCCA | TCGAGCGAACGACTTGCA | 59.65 | 59.67 | 181 | 135 |
100.00%
|
100.00%
|
| Primer 4 | Prevotella ruminicola | TTGCCAGCACCTGCAGAA | AATCGCCAAGACGTCGCT | 59.80 | 59.74 | 225 | 128 |
100.00%
|
100.00%
|
| Primer 5 | Prevotella ruminicola | GTTAGGTAAAGCGCACGGC | ACAATGGCTGCCCACGAA | 59.58 | 59.88 | 279 | 128 |
100.00%
|
100.00%
|
| Primer 6 | Prevotella ruminicola | ACGTGTTCGACCGCACA | ACAATGGCTGCCCACGAA | 59.53 | 59.88 | 226 | 128 |
100.00%
|
100.00%
|
| Primer 7 | Prevotella ruminicola | CGTGTTCGACCGCACAGAT | ACAATGGCTGCCCACGAA | 60.73 | 59.88 | 225 | 128 |
100.00%
|
100.00%
|
| Primer 8 | Prevotella ruminicola | GTGTTCGACCGCACAGATG | ACAATGGCTGCCCACGAA | 59.22 | 59.88 | 224 | 128 |
100.00%
|
100.00%
|
| Primer 9 | Prevotella ruminicola | TTCGTCGTGAGCGCATGT | GCCTTCAGCAGTGCGAAAC | 60.05 | 60.08 | 241 | 126 |
100.00%
|
100.00%
|
| Primer 10 | Prevotella ruminicola | GCTGAAGAGCTACGACCACA | TCAACCTCAACACCGCTGG | 59.76 | 60.23 | 270 | 126 |
100.00%
|
100.00%
|