| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1211 | Priestia megaterium | TTGGTGGAATGGGATCGCC | GCATCGGTTTGCGTGCTT | 60.08 | 59.74 | 172 | 65 |
100.00%
|
100.00%
|
| Primer 1212 | Priestia megaterium | AGCTGCTGATGTACTGGGG | TCGACAGTTCCAGCCGTT | 59.09 | 58.87 | 273 | 65 |
100.00%
|
100.00%
|
| Primer 1213 | Priestia megaterium | AGCTGCTGATGTACTGGGG | TCGACAGTTCCAGCCGTTT | 59.09 | 59.56 | 273 | 65 |
100.00%
|
100.00%
|
| Primer 1214 | Priestia megaterium | AGCTGCTGATGTACTGGGG | ATCGACAGTTCCAGCCGTT | 59.09 | 59.33 | 274 | 65 |
100.00%
|
100.00%
|
| Primer 1215 | Priestia megaterium | CAGGCTTTTGGATGCGCTT | CGAAACAGCACAACAGGCC | 59.41 | 60.01 | 274 | 65 |
100.00%
|
100.00%
|
| Primer 1216 | Priestia megaterium | CAGGCTTTTGGATGCGCTT | ACAGCACAACAGGCCAAGT | 59.41 | 60.08 | 270 | 65 |
100.00%
|
100.00%
|
| Primer 1217 | Priestia megaterium | AGCAAGCCAAGACCAGGTC | ACGAAACAACAGCAGCGC | 59.93 | 59.67 | 293 | 65 |
100.00%
|
100.00%
|
| Primer 1218 | Priestia megaterium | GAGCAAGCCAAGACCAGGT | ACGAAACAACAGCAGCGC | 59.93 | 59.67 | 294 | 65 |
100.00%
|
100.00%
|
| Primer 1219 | Priestia megaterium | AGCAAGCCAAGACCAGGT | ACGAAACAACAGCAGCGC | 58.74 | 59.67 | 293 | 65 |
100.00%
|
100.00%
|
| Primer 1220 | Priestia megaterium | TGGTATGCTTTGCAGGGCTT | TGAGGGAATGCGCTGTGA | 60.25 | 58.93 | 156 | 65 |
100.00%
|
100.00%
|