| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1541 | Priestia megaterium | TGAGCGGACATACACACGG | TGCGTTTGGGCTCTAGCTT | 59.79 | 59.63 | 157 | 55 |
100.00%
|
100.00%
|
| Primer 1542 | Priestia megaterium | CCTGATGCCACTCCGGAAT | GCATCGGTTTGCGTGCTT | 59.47 | 59.74 | 154 | 55 |
100.00%
|
100.00%
|
| Primer 1543 | Priestia megaterium | CCAATCGGAGCAGACCAAGA | TCGCCTTCTTCGTCAAGGAA | 59.75 | 59.32 | 164 | 55 |
100.00%
|
100.00%
|
| Primer 1544 | Priestia megaterium | CCAATCGGAGCAGACCAAGA | TTCGCCTTCTTCGTCAAGGA | 59.75 | 59.32 | 165 | 55 |
100.00%
|
100.00%
|
| Primer 1545 | Priestia megaterium | TTTCACCGCTGCGTAACG | CGCGTTTTGCAGGCTCTT | 58.76 | 59.36 | 168 | 55 |
100.00%
|
100.00%
|
| Primer 1546 | Priestia megaterium | TCCGTATGGGAAGCATGGG | AAGCTTGCTGACACGCCT | 59.16 | 59.89 | 244 | 55 |
100.00%
|
100.00%
|
| Primer 1547 | Priestia megaterium | TCAGCGGTATCGTCGGAGA | ACCGACTAGCTGAGAGGCA | 60.15 | 60.00 | 272 | 55 |
100.00%
|
100.00%
|
| Primer 1548 | Priestia megaterium | TCAGCGGTATCGTCGGAGA | AGGCATCAGCTGGTGTCAC | 60.15 | 60.00 | 258 | 55 |
100.00%
|
100.00%
|
| Primer 1549 | Priestia megaterium | TCAGCGGTATCGTCGGAGA | GAGGCATCAGCTGGTGTCA | 60.15 | 59.70 | 259 | 55 |
100.00%
|
100.00%
|
| Primer 1550 | Priestia megaterium | TCAGCGGTATCGTCGGAGA | GGCATCAGCTGGTGTCACA | 60.15 | 60.30 | 257 | 55 |
100.00%
|
100.00%
|