| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 221 | Priestia megaterium | GCGGTGTGATGCTAGAGGA | ACGAGCTTCGCCTGCATT | 59.18 | 60.05 | 221 | 102 |
100.00%
|
100.00%
|
| Primer 222 | Priestia megaterium | CACCAGCTCGTGCAAAAGT | ACGAGCTTCGCCTGCATT | 58.98 | 60.05 | 191 | 102 |
100.00%
|
100.00%
|
| Primer 223 | Priestia megaterium | GGTGTGATGCTAGAGGACGG | ACGAGCTTCGCCTGCATT | 59.90 | 60.05 | 219 | 102 |
100.00%
|
100.00%
|
| Primer 224 | Priestia megaterium | CGGTGTGATGCTAGAGGACG | ACGAGCTTCGCCTGCATT | 60.25 | 60.05 | 220 | 102 |
100.00%
|
100.00%
|
| Primer 225 | Priestia megaterium | TTAGGCGTACGCGAACCA | GGTGGCGAAACTCTTCACG | 59.04 | 59.14 | 295 | 102 |
100.00%
|
100.00%
|
| Primer 226 | Priestia megaterium | AGTGCAGCTGGTCATGAGG | ACAGAAGCGCTTGAGGTGA | 59.70 | 59.25 | 151 | 102 |
100.00%
|
100.00%
|
| Primer 227 | Priestia megaterium | AGTGCAGCTGGTCATGAGG | AACAGAAGCGCTTGAGGTGA | 59.70 | 59.89 | 152 | 102 |
100.00%
|
100.00%
|
| Primer 228 | Priestia megaterium | TTGCACCCAGCTGCTTCA | CGTTTGCGGATGATGGAGC | 59.80 | 59.65 | 244 | 102 |
100.00%
|
100.00%
|
| Primer 229 | Priestia megaterium | TTGCACCCAGCTGCTTCA | GCGTTTGCGGATGATGGAG | 59.80 | 59.65 | 245 | 102 |
100.00%
|
100.00%
|
| Primer 230 | Priestia megaterium | AGATAGCCCTGACCACCGT | GGCCTTTGGTAGCGCTGTA | 60.00 | 60.08 | 233 | 102 |
100.00%
|
100.00%
|