| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 61 | Pseudomonas fragi | ATGAGCGCATCACTGCCT | CCGATAAAGGGCAGCAGGT | 59.41 | 59.78 | 251 | 92 |
100.00%
|
100.00%
|
| Primer 62 | Pseudomonas fragi | ATGAGCGCATCACTGCCT | AAGGGCAGCAGGTGATGTT | 59.41 | 59.54 | 245 | 92 |
100.00%
|
100.00%
|
| Primer 63 | Pseudomonas fragi | ATGAGCGCATCACTGCCT | AAAGGGCAGCAGGTGATGT | 59.41 | 59.54 | 246 | 92 |
100.00%
|
100.00%
|
| Primer 64 | Pseudomonas fragi | TCGCAGCTTGGTGATGGT | GCGCACAGGTTACAGGCTA | 59.25 | 60.08 | 159 | 92 |
100.00%
|
100.00%
|
| Primer 65 | Pseudomonas fragi | GCCTGTATGTGGCCTGGTT | GCCGTCCTGCTGTTCAAAC | 60.00 | 59.72 | 152 | 92 |
100.00%
|
100.00%
|
| Primer 66 | Pseudomonas fragi | ATTGCTGTTGCCGTGTGC | ACGCAACACCAGCACGAT | 59.66 | 60.28 | 201 | 91 |
100.00%
|
100.00%
|
| Primer 67 | Pseudomonas fragi | CGGACGTGACGATGACCAA | TGGGCAGCTCGCTTTTGA | 60.08 | 59.89 | 153 | 90 |
100.00%
|
100.00%
|
| Primer 68 | Pseudomonas fragi | TGCGCTGTTGTTGCTGGA | GCTGCCTTCGATGACCAGT | 60.52 | 60.08 | 232 | 90 |
100.00%
|
100.00%
|
| Primer 69 | Pseudomonas fragi | CCCAAGGAAGGCAAGTCGA | AGCGGATGTCAGCGGTTT | 59.63 | 59.65 | 163 | 89 |
100.00%
|
100.00%
|
| Primer 70 | Pseudomonas fragi | TGCAAGTTCCAGCAGCGA | TTCCTCCCACTTGCCCTCT | 59.89 | 60.15 | 208 | 89 |
100.00%
|
100.00%
|