| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Ralstonia pickettii | TGGCCTCACACGGTTTGA | TGAACAGCAGGATCACCGC | 59.09 | 60.38 | 228 | 125 |
100.00%
|
100.00%
|
| Primer 42 | Ralstonia pickettii | ATCCTGCGCACGAAGAAGG | CGTGAACACCTTGTCGTGC | 60.45 | 59.72 | 156 | 124 |
100.00%
|
50.00%
|
| Primer 43 | Ralstonia pickettii | CATTGCCGTGAACGTGGTG | ACGCTGCGCATTGATCCA | 60.08 | 60.44 | 193 | 124 |
100.00%
|
50.00%
|
| Primer 44 | Ralstonia pickettii | ATTGCCGTGAACGTGGTG | ACGCTGCGCATTGATCCA | 58.66 | 60.44 | 192 | 124 |
100.00%
|
50.00%
|
| Primer 45 | Ralstonia pickettii | TCGATGGATGCGCTGCAA | AGCATCAGTCCGATCAGCG | 60.13 | 59.93 | 200 | 124 |
100.00%
|
50.00%
|
| Primer 46 | Ralstonia pickettii | GCGACACCGTGAAGAAAGC | TTGACGCTTTTGCCGTGC | 59.80 | 59.97 | 277 | 124 |
100.00%
|
50.00%
|
| Primer 47 | Ralstonia pickettii | ACGCAACGCAATCTTCCG | GCGTGAATACCATGTGCCG | 59.14 | 59.65 | 211 | 124 |
100.00%
|
50.00%
|
| Primer 48 | Ralstonia pickettii | TGCGCAATCACGGCTACA | TACAGAATGCCCTGCTGCG | 60.05 | 60.45 | 289 | 124 |
100.00%
|
50.00%
|
| Primer 49 | Ralstonia pickettii | ACGGCTGCTGATTGCGTA | TCTTCGGTGATCCACGTGC | 59.74 | 60.08 | 258 | 122 |
100.00%
|
50.00%
|
| Primer 50 | Ralstonia pickettii | ACGGCTGCTGATTGCGTA | TCGATGCGGATTTCCCAGG | 59.74 | 59.85 | 180 | 122 |
100.00%
|
50.00%
|