| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 501 | Ralstonia pickettii | ATTCCACGAACGTGCGGT | TTCGTCGAGCTCCAGCAA | 59.97 | 58.95 | 250 | 72 |
100.00%
|
50.00%
|
| Primer 502 | Ralstonia pickettii | TCCGGACAAGCCAAGCAT | TGGTGCATTTCCAGCGCT | 59.24 | 60.28 | 183 | 72 |
100.00%
|
50.00%
|
| Primer 503 | Ralstonia pickettii | ACAGGCTTTGGCGATGCT | TCACCGCATCGTGCACAT | 59.97 | 60.05 | 203 | 72 |
100.00%
|
50.00%
|
| Primer 504 | Ralstonia pickettii | ACAGGCTTTGGCGATGCT | ATCACCGCATCGTGCACA | 59.97 | 60.05 | 204 | 72 |
100.00%
|
50.00%
|
| Primer 505 | Ralstonia pickettii | TCGGCTTGCGCAACATGA | TTGCTGCCCAACCAAGGT | 60.67 | 59.72 | 166 | 72 |
100.00%
|
50.00%
|
| Primer 506 | Ralstonia pickettii | TGACAGTGGCATTCGGCT | TTGCTGCCCAACCAAGGT | 59.25 | 59.72 | 178 | 72 |
100.00%
|
50.00%
|
| Primer 507 | Ralstonia pickettii | TCGATGTGGTGACAGTGGC | TTGCTGCCCAACCAAGGT | 60.01 | 59.72 | 187 | 72 |
100.00%
|
50.00%
|
| Primer 508 | Ralstonia pickettii | AAATGGTGCTCGGCTGCA | GGCAGGATGACGTTCAGCT | 60.28 | 60.08 | 223 | 72 |
100.00%
|
50.00%
|
| Primer 509 | Ralstonia pickettii | CCGCTGCGCATTTTCGAA | TGCCACGCACCTTGATCA | 59.82 | 59.57 | 208 | 72 |
100.00%
|
50.00%
|
| Primer 510 | Ralstonia pickettii | AACGTCGCCAAGGCCAAT | AGTTGGCGTCGTTGGTGT | 60.28 | 59.81 | 277 | 72 |
100.00%
|
50.00%
|