| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Rhizobium etli | TCGGTTTGCGCTATGGCA | GCAAGGGCAAAGCGACAT | 60.05 | 59.04 | 294 | 76 |
100.00%
|
25.00%
|
| Primer 12 | Rhizobium etli | AAGGCATCGACCTGCACA | TGAACCGGATGATCGCGAC | 59.25 | 60.23 | 201 | 75 |
100.00%
|
25.00%
|
| Primer 13 | Rhizobium etli | TCGGTTTGCGCTATGGCA | ATGGTCGAGCAGGCGAAAA | 60.05 | 60.00 | 260 | 75 |
100.00%
|
25.00%
|
| Primer 14 | Rhizobium etli | ATTGCGCTTGTCATCGGC | ATGGACCAGAAGCCGAACC | 59.21 | 59.70 | 245 | 74 |
100.00%
|
25.00%
|
| Primer 15 | Rhizobium etli | CATCGAGCGCATTGAACGG | TTGCTTCCTCCTCCATGCG | 60.01 | 60.08 | 164 | 74 |
100.00%
|
25.00%
|
| Primer 16 | Rhizobium etli | AACTACGTCGTGGTGGTCG | AAAGCGCTCTCTGCACCA | 59.72 | 59.57 | 201 | 74 |
100.00%
|
25.00%
|
| Primer 17 | Rhizobium etli | AAGGATCAGCAGCTGACCG | TGGCGTTGCGAATTGCTG | 59.78 | 59.75 | 171 | 73 |
100.00%
|
25.00%
|
| Primer 18 | Rhizobium etli | ACCGCACGCATGGACAAA | AAAAGGGCATGCCGAGCT | 60.59 | 59.96 | 163 | 72 |
100.00%
|
25.00%
|
| Primer 19 | Rhizobium etli | TGGACAAACGCTTTGCCG | AAAAGGGCATGCCGAGCT | 59.28 | 59.96 | 153 | 72 |
100.00%
|
25.00%
|
| Primer 20 | Rhizobium etli | CACGCATGGACAAACGCT | AAAAGGGCATGCCGAGCT | 59.05 | 59.96 | 159 | 72 |
100.00%
|
25.00%
|