| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 231 | Rhizobium etli | TTTCGCTCGTCATGGCCA | TCTTGCGGAACATGGCGA | 59.65 | 59.65 | 282 | 28 |
100.00%
|
25.00%
|
| Primer 232 | Rhizobium etli | TGCTGCACCATATCGGCA | ATTGGCGACGGTCACCTT | 59.41 | 59.25 | 200 | 25 |
100.00%
|
25.00%
|
| Primer 233 | Rhizobium etli | TTCGCTCGGCATGGATGT | AACCATGGAGGCAAGCGT | 59.42 | 59.56 | 154 | 24 |
100.00%
|
25.00%
|
| Primer 234 | Rhizobium etli | GCGCAACCAAATCGCCAT | AGGTCGAAAAGCGCACGT | 59.82 | 60.28 | 164 | 24 |
100.00%
|
25.00%
|
| Primer 235 | Rhizobium etli | ATGCCGTGGACAATGCCT | CGTATTTCTTGGCGTCGCG | 59.64 | 60.01 | 186 | 22 |
100.00%
|
25.00%
|
| Primer 236 | Rhizobium etli | TTTCCTGATGCGCACCGA | CGGTCGGCATCAATCTCGA | 59.65 | 59.93 | 180 | 21 |
100.00%
|
25.00%
|
| Primer 237 | Rhizobium etli | TGGCTGCCATGTCAACGA | AGTTGCGGATGGCCTGTT | 59.57 | 59.56 | 195 | 21 |
100.00%
|
25.00%
|
| Primer 238 | Rhizobium etli | TGGCTGCCATGTCAACGA | AAGTTGCGGATGGCCTGT | 59.57 | 59.56 | 196 | 21 |
100.00%
|
25.00%
|
| Primer 239 | Rhizobium etli | ACCTCGCAGCACATCGAA | ATGTTGGAGGCATCGCGA | 59.34 | 59.42 | 266 | 21 |
100.00%
|
25.00%
|
| Primer 240 | Rhizobium etli | TTTCCTCGGCATCTGCGT | AGCGTGTTCCAGCCGATT | 59.34 | 59.65 | 153 | 18 |
100.00%
|
25.00%
|