| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Rhizobium leguminosarum | ATGCGAACCAGCTGCTGT | GTGAACCAGATCGAGCCGT | 59.97 | 59.78 | 277 | 35 |
100.00%
|
100.00%
|
| Primer 32 | Rhizobium leguminosarum | GAAGAGCTTCACCACGGCT | AAAGCTCTTGGCCAGCGT | 60.00 | 59.89 | 172 | 35 |
100.00%
|
100.00%
|
| Primer 33 | Rhizobium leguminosarum | AGAAGAGCTTCACCACGGC | AAAGCTCTTGGCCAGCGT | 60.00 | 59.89 | 173 | 35 |
100.00%
|
100.00%
|
| Primer 34 | Rhizobium leguminosarum | AAGAGCTTCACCACGGCT | AAAGCTCTTGGCCAGCGT | 58.85 | 59.89 | 171 | 35 |
100.00%
|
100.00%
|
| Primer 35 | Rhizobium leguminosarum | ACGCTGGCCAAGAGCTTT | TCTCCATCGTCGAGAGCGA | 59.89 | 60.15 | 203 | 32 |
100.00%
|
100.00%
|
| Primer 36 | Rhizobium leguminosarum | ATCCGCAACCTGCCGATT | ACAGTGATGGCAGCACGT | 59.73 | 59.57 | 265 | 27 |
100.00%
|
100.00%
|
| Primer 37 | Rhizobium leguminosarum | ACGTCTTTCGCAACCCGA | ATGCAGCGTCAGGTTCGT | 59.58 | 59.66 | 254 | 24 |
100.00%
|
100.00%
|
| Primer 38 | Rhizobium leguminosarum | AGATCGGCTTGCGCATGT | AACCCTTCAGTCCCGTCGA | 60.12 | 60.53 | 153 | 24 |
100.00%
|
50.00%
|
| Primer 39 | Rhizobium leguminosarum | AAGCCACGACGAAAGCCT | TCGGGTCGAAGCAATGCA | 59.57 | 59.65 | 179 | 22 |
100.00%
|
100.00%
|
| Primer 40 | Rhizobium leguminosarum | CGCAACCCGTGAAAAGCT | TCGGGTTGCGAAAGACGT | 58.97 | 59.58 | 200 | 20 |
100.00%
|
50.00%
|