| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Rickettsia felis | AGCAAAGTGTTGGTCAGCTG | CAAGCCGAGAAGCAACTGC | 58.97 | 59.79 | 173 | 138 |
100.00%
|
100.00%
|
| Primer 2 | Rickettsia felis | TGCAACGCCTTTAGCTGC | TGCTGCACGTTTTCTACATGC | 59.05 | 60.07 | 204 | 128 |
100.00%
|
100.00%
|
| Primer 3 | Rickettsia felis | ACAGCAGTTTGGGCTAAGAAA | ACGTTGGCACATTCCCCTC | 58.06 | 60.30 | 232 | 127 |
100.00%
|
100.00%
|
| Primer 4 | Rickettsia felis | ACAGCAGTTTGGGCTAAGAAA | TTGGCACATTCCCCTCTCC | 58.06 | 59.31 | 229 | 127 |
100.00%
|
100.00%
|
| Primer 5 | Rickettsia felis | ACAGCAGTTTGGGCTAAGAAA | AACGTTGGCACATTCCCC | 58.06 | 58.23 | 233 | 127 |
100.00%
|
100.00%
|
| Primer 6 | Rickettsia felis | ACAGCAGTTTGGGCTAAGAAA | CACATTCCCCTCTCCCGATG | 58.06 | 59.89 | 225 | 127 |
100.00%
|
100.00%
|
| Primer 7 | Rickettsia felis | TGCCCTTGGTGTGGTACT | TCCACGCTTCTTCCACCA | 58.00 | 58.44 | 156 | 125 |
100.00%
|
100.00%
|
| Primer 8 | Rickettsia felis | GCAACGCCTTTAGCTGCTC | TGCTGCACGTTTTCTACATGC | 59.86 | 60.07 | 203 | 120 |
100.00%
|
100.00%
|
| Primer 9 | Rickettsia felis | TGGAGCTAGCAATACTTGGCA | CCCCATTGCTTCTGCCCAA | 59.44 | 60.61 | 215 | 120 |
100.00%
|
100.00%
|
| Primer 10 | Rickettsia felis | TTGCAACGCCTTTAGCTGC | TGCTGCACGTTTTCTACATGC | 59.71 | 60.07 | 205 | 118 |
100.00%
|
100.00%
|