| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 101 | Rickettsia felis | TCGAGTACCGGATCGACGT | CCACTTGCTGTTTCGGCAT | 60.15 | 59.04 | 266 | 82 |
100.00%
|
100.00%
|
| Primer 102 | Rickettsia felis | TCGAGTACCGGATCGACGT | AAACCACTTGCTGTTTCGGC | 60.15 | 59.90 | 269 | 82 |
100.00%
|
100.00%
|
| Primer 103 | Rickettsia felis | TCGAGTACCGGATCGACGT | CCACTTGCTGTTTCGGCATC | 60.15 | 60.11 | 266 | 82 |
100.00%
|
100.00%
|
| Primer 104 | Rickettsia felis | AGCAAAGTGTTGGTCAGCTGA | CAAGCCGAGAAGCAACTGC | 60.41 | 59.79 | 173 | 82 |
100.00%
|
100.00%
|
| Primer 105 | Rickettsia felis | ACCCTAATTCCTTACGCAGCA | AAGCCACTGAGCGGTGCTA | 59.44 | 61.58 | 219 | 82 |
100.00%
|
100.00%
|
| Primer 106 | Rickettsia felis | CTTCTCCTGCAGAAACCGGA | GACGGAGGAGCTTCAAAATGA | 59.68 | 58.30 | 220 | 82 |
100.00%
|
100.00%
|
| Primer 107 | Rickettsia felis | TGCCCTTGGTGTGGTACT | ACGCTTCTTCCACCATTCGT | 58.00 | 59.97 | 153 | 82 |
100.00%
|
100.00%
|
| Primer 108 | Rickettsia felis | GGCACGTGCAGAATTAGCTG | AGCCCTGTGACCGATTTTGAT | 59.90 | 60.00 | 211 | 81 |
100.00%
|
100.00%
|
| Primer 109 | Rickettsia felis | CTTCTCCTGCAGAAACCGGA | AGACGGAGGAGCTTCAAAATG | 59.68 | 58.30 | 221 | 80 |
100.00%
|
100.00%
|
| Primer 110 | Rickettsia felis | AGCCCAGTTAACCCAACAGC | ACCCTCGCAAAACTCGTTTT | 60.54 | 58.61 | 161 | 80 |
100.00%
|
100.00%
|