| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 171 | Rickettsia felis | TTGCGTTGACGTATGCCCT | TCAGGCAAGGCAGGCTTT | 60.00 | 59.48 | 222 | 70 |
100.00%
|
100.00%
|
| Primer 172 | Rickettsia felis | TGCGTTGACGTATGCCCT | ATCAGGCAAGGCAGGCTTT | 59.34 | 59.92 | 222 | 70 |
100.00%
|
100.00%
|
| Primer 173 | Rickettsia felis | TGCGTTGACGTATGCCCT | TCAGGCAAGGCAGGCTTTT | 59.34 | 60.15 | 221 | 70 |
100.00%
|
100.00%
|
| Primer 174 | Rickettsia felis | TGACCGATCCGATAGCAGC | AGACGCAAAGCTGCCCAA | 59.34 | 60.20 | 235 | 70 |
100.00%
|
100.00%
|
| Primer 175 | Rickettsia felis | ACCGATCCGATAGCAGCA | AGACGCAAAGCTGCCCAA | 58.14 | 60.20 | 233 | 70 |
100.00%
|
100.00%
|
| Primer 176 | Rickettsia felis | ACGTGGTGAGCGTGCTTAT | TTCTCGCCTCCGCAAAGT | 59.71 | 59.26 | 291 | 69 |
100.00%
|
100.00%
|
| Primer 177 | Rickettsia felis | TGCAACGCCTTTAGCTGC | GCTGCACGTTTTCTACATGCT | 59.05 | 59.80 | 203 | 68 |
100.00%
|
100.00%
|
| Primer 178 | Rickettsia felis | GCAACGCCTTTAGCTGCTC | GCTGCACGTTTTCTACATGCT | 59.86 | 59.80 | 202 | 68 |
100.00%
|
100.00%
|
| Primer 179 | Rickettsia felis | TGAGCGTGGAGAAACAGCA | GGAGAATGCCCACAGAAGC | 59.55 | 58.51 | 222 | 68 |
100.00%
|
100.00%
|
| Primer 180 | Rickettsia felis | ATGAGCGTGGAGAAACAGCA | GGAGAATGCCCACAGAAGCT | 59.96 | 60.03 | 223 | 68 |
100.00%
|
100.00%
|