| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 51 | Rickettsia felis | AAAGGCTTGCGTACCGGT | GACCGTCTGTTGCTGGAGT | 59.57 | 59.63 | 214 | 98 |
100.00%
|
100.00%
|
| Primer 52 | Rickettsia felis | AAAGGCTTGCGTACCGGT | TGACCGTCTGTTGCTGGA | 59.57 | 58.46 | 215 | 98 |
100.00%
|
100.00%
|
| Primer 53 | Rickettsia felis | AAAGGCTTGCGTACCGGT | ACCGTCTGTTGCTGGAGT | 59.57 | 58.45 | 213 | 98 |
100.00%
|
100.00%
|
| Primer 54 | Rickettsia felis | AAAGGCTTGCGTACCGGT | TTGACCGTCTGTTGCTGGA | 59.57 | 59.17 | 216 | 98 |
100.00%
|
100.00%
|
| Primer 55 | Rickettsia felis | GAAGCATCCGACGCTAAAACT | TAGCCGTGTTGCATCGCA | 59.00 | 60.05 | 176 | 98 |
100.00%
|
100.00%
|
| Primer 56 | Rickettsia felis | TGGAGCTAGCAATACTTGGCA | CCCATTGCTTCTGCCCAAT | 59.44 | 58.40 | 214 | 98 |
100.00%
|
100.00%
|
| Primer 57 | Rickettsia felis | CGCAGCTTTAGGGGTTGCT | ACAGGCCATGCACAATCCA | 60.68 | 59.92 | 273 | 98 |
100.00%
|
100.00%
|
| Primer 58 | Rickettsia felis | TTTACGACGCGTTGGGGA | ACGCTCCTTGCTGCTTCT | 59.27 | 59.25 | 187 | 97 |
100.00%
|
100.00%
|
| Primer 59 | Rickettsia felis | CGGTATGAGCGATGCACTG | TCAGCAGCAGCCGTGAAA | 58.78 | 59.89 | 240 | 97 |
100.00%
|
100.00%
|
| Primer 60 | Rickettsia felis | GGCACGTGCAGAATTAGCTG | AGCCCTGTGACCGATTTTG | 59.90 | 58.05 | 211 | 93 |
100.00%
|
100.00%
|