| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Rickettsia typhi | CAGGAAGCAAAAGCGGTGG | ACATCTGCACTGACCGGTG | 59.71 | 60.00 | 244 | 117 |
100.00%
|
100.00%
|
| Primer 12 | Rickettsia typhi | GCAGGAAGCAAAAGCGGTG | ACATCTGCACTGACCGGTG | 60.37 | 60.00 | 245 | 117 |
100.00%
|
100.00%
|
| Primer 13 | Rickettsia typhi | TGGGAACAACAGCAGCACA | CGTAGGACGTGTTTCCGGT | 60.08 | 59.71 | 257 | 117 |
100.00%
|
100.00%
|
| Primer 14 | Rickettsia typhi | TGGGCAACATGGGCAGAA | GGGATACTAACTGCCTCGCA | 59.47 | 59.25 | 251 | 117 |
100.00%
|
100.00%
|
| Primer 15 | Rickettsia typhi | TGGGCAACATGGGCAGAA | TGGGATACTAACTGCCTCGC | 59.47 | 59.25 | 252 | 114 |
100.00%
|
100.00%
|
| Primer 16 | Rickettsia typhi | CCTCGTTCAGGGCTTGCAA | AAAGCCACCTCTACCACGC | 60.60 | 60.00 | 231 | 114 |
100.00%
|
100.00%
|
| Primer 17 | Rickettsia typhi | GCCAGGCTTAATGCTTAAGGT | CACGGGAAAGCTATAGTTGCG | 58.62 | 59.41 | 298 | 113 |
100.00%
|
100.00%
|
| Primer 18 | Rickettsia typhi | ATCGCTGGCAGAGGTTCAC | TCGCCTCGAATTGTTTGTCC | 60.08 | 58.85 | 296 | 113 |
100.00%
|
100.00%
|
| Primer 19 | Rickettsia typhi | TGGGAACAACAGCAGCACA | TCAGGCGTAGGACGTGTT | 60.08 | 58.23 | 262 | 111 |
100.00%
|
100.00%
|
| Primer 20 | Rickettsia typhi | ATGCTGGGCAAAGCGCTA | GCCATGCTTCCCAATCACG | 60.05 | 59.57 | 270 | 111 |
100.00%
|
100.00%
|