| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 461 | Roseburia intestinalis | GACGGCATTGCAGCAGTT | TCGCCACGGCATTTTCCT | 59.04 | 59.97 | 254 | 82 |
100.00%
|
50.00%
|
| Primer 462 | Roseburia intestinalis | TTCCGCTTGCAGCAGTCA | TGTCACATGACCGGACACG | 59.89 | 60.01 | 182 | 82 |
100.00%
|
50.00%
|
| Primer 463 | Roseburia intestinalis | AAACAGTCGCTGCTCGCA | ACAGGCGTGCCACAATCA | 60.28 | 59.89 | 190 | 82 |
100.00%
|
50.00%
|
| Primer 464 | Roseburia intestinalis | TGCTCGCATGGACACAGT | ACAGGCGTGCCACAATCA | 59.26 | 59.89 | 180 | 82 |
100.00%
|
50.00%
|
| Primer 465 | Roseburia intestinalis | AATGCTGCACCTCGCCAT | GTAAGCGGAACCGTGGTCA | 60.04 | 60.01 | 202 | 82 |
100.00%
|
50.00%
|
| Primer 466 | Roseburia intestinalis | AGCCTGTTTGAGTCGGCA | TTGCACCACCGTTTGCCT | 59.17 | 60.44 | 238 | 82 |
100.00%
|
50.00%
|
| Primer 467 | Roseburia intestinalis | AACCGCTCATGCTCGTGA | CACACTGCAGCAGTTCCCT | 59.34 | 60.23 | 177 | 82 |
100.00%
|
50.00%
|
| Primer 468 | Roseburia intestinalis | TGCAGGAACTTCGCGTGA | ATCGCCATACAGCCAGCA | 59.58 | 59.09 | 179 | 82 |
100.00%
|
50.00%
|
| Primer 469 | Roseburia intestinalis | ACACAGACCCGTGAACTGC | TCACTGACAGAGCACATGGAC | 60.23 | 60.00 | 161 | 81 |
100.00%
|
50.00%
|
| Primer 470 | Roseburia intestinalis | TCGCAGCCATTCAGAGAGC | ATGCCGTCAGGTTCCGAT | 60.15 | 58.69 | 279 | 81 |
100.00%
|
50.00%
|