| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 221 | Roseobacter denitrificans | CGGTCTTGCCCATCGTGAT | AGCAGCGCAAGACCGAAT | 60.15 | 60.05 | 196 | 106 |
100.00%
|
100.00%
|
| Primer 222 | Roseobacter denitrificans | TCTGATTTCGCGTGCGGT | TGTTTGTACCGCTGGCCA | 60.05 | 59.49 | 230 | 106 |
100.00%
|
100.00%
|
| Primer 223 | Roseobacter denitrificans | TGCTGGTGCATCGCAATG | AGCCACCGCACATGTGAA | 59.12 | 59.89 | 297 | 106 |
100.00%
|
100.00%
|
| Primer 224 | Roseobacter denitrificans | ACGCATGAATGTCTGGGCT | AGCCACCGCACATGTGAA | 59.70 | 59.89 | 169 | 106 |
100.00%
|
100.00%
|
| Primer 225 | Roseobacter denitrificans | CACATGTCCCACACGAGCT | TGCGCATCTCCAGCATGT | 60.00 | 59.73 | 183 | 106 |
100.00%
|
100.00%
|
| Primer 226 | Roseobacter denitrificans | TTGCGCTTGCGCTTACAG | ACGCAACTACGCCAGGAA | 59.44 | 59.26 | 195 | 106 |
100.00%
|
100.00%
|
| Primer 227 | Roseobacter denitrificans | ACCACCAAGGGTTACGGGT | AAGATCGCTCGCCATCTGG | 60.77 | 59.93 | 165 | 106 |
100.00%
|
100.00%
|
| Primer 228 | Roseobacter denitrificans | TCGGACATGGCCAAAGCT | TGCAGTCGCTTCGTTGGT | 59.24 | 59.89 | 222 | 106 |
100.00%
|
100.00%
|
| Primer 229 | Roseobacter denitrificans | ACCGCAGCAGAAAAGTCCA | TGCAGTCGCTTCGTTGGT | 59.85 | 59.89 | 285 | 106 |
100.00%
|
100.00%
|
| Primer 230 | Roseobacter denitrificans | CAGCAGTTCCTCGAAGGGT | TGTCGCCATTGTCCAGCA | 59.33 | 59.57 | 157 | 106 |
100.00%
|
100.00%
|