| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 251 | Rothia aeria | AACCGCAGAAACCGCTGA | GCTCATCGGCTGGGAATGA | 59.89 | 59.85 | 236 | 68 |
100.00%
|
100.00%
|
| Primer 252 | Rothia aeria | TGGCTCATCCTACCCGTGA | AATCGCCTGCCCATCGTT | 60.00 | 59.73 | 254 | 67 |
100.00%
|
100.00%
|
| Primer 253 | Rothia aeria | CGCATGTTTCGCTCACTGG | AATCGCCTGCCCATCGTT | 59.87 | 59.73 | 164 | 67 |
100.00%
|
100.00%
|
| Primer 254 | Rothia aeria | GCATGTTTCGCTCACTGGC | AATCGCCTGCCCATCGTT | 60.15 | 59.73 | 163 | 67 |
100.00%
|
100.00%
|
| Primer 255 | Rothia aeria | AACTGGTCAGCAGCCGAA | TGCGTTTAGGGTGGCGTT | 59.17 | 59.89 | 223 | 67 |
100.00%
|
100.00%
|
| Primer 256 | Rothia aeria | ACGGTTTCTGAGTCCGCTG | CCCGTCTGAATCGTTCCGT | 60.01 | 59.79 | 230 | 67 |
100.00%
|
100.00%
|
| Primer 257 | Rothia aeria | AAGCTGCTGTACTGGCTGG | TCCCAGCCATTTGCCCAT | 60.00 | 59.22 | 156 | 67 |
100.00%
|
100.00%
|
| Primer 258 | Rothia aeria | GGGCTGCTGCTGGTTATGA | AACATGGTTGCCCAGCGT | 60.08 | 60.20 | 210 | 67 |
100.00%
|
100.00%
|
| Primer 259 | Rothia aeria | AAACCACGCTCCTGCACA | CGTTGTCGGTTTCGCGTT | 59.81 | 59.38 | 213 | 67 |
100.00%
|
100.00%
|
| Primer 260 | Rothia aeria | ATGTGAACGCTGCCTCGT | ACAAACTCCAGCAGGGCTC | 59.66 | 59.93 | 286 | 67 |
100.00%
|
100.00%
|