| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Rothia aeria | TGCCATTGCCGTTCAGCT | GCTCGTTAACACGCTGCA | 60.28 | 58.76 | 287 | 110 |
100.00%
|
100.00%
|
| Primer 32 | Rothia aeria | TGCCATTGCCGTTCAGCT | AGCTCGTTAACACGCTGCA | 60.28 | 60.30 | 288 | 110 |
100.00%
|
100.00%
|
| Primer 33 | Rothia aeria | TGGGTCACCGCTGATTTGG | ACGCAAATCCCAGGTGCT | 60.30 | 59.56 | 291 | 110 |
100.00%
|
100.00%
|
| Primer 34 | Rothia aeria | TCAGGACGGGTTTGAGCTG | ACGCAAATCCCAGGTGCT | 59.63 | 59.56 | 232 | 110 |
100.00%
|
100.00%
|
| Primer 35 | Rothia aeria | GTCAGGACGGGTTTGAGCT | ACGCAAATCCCAGGTGCT | 59.63 | 59.56 | 233 | 110 |
100.00%
|
100.00%
|
| Primer 36 | Rothia aeria | TGAGCGCACCCGTTTTCT | TGTTGCTTCAACGGTGCC | 59.89 | 58.88 | 179 | 109 |
100.00%
|
100.00%
|
| Primer 37 | Rothia aeria | TGAGCGCACCCGTTTTCT | TGATACGCACCCTGATGCC | 59.89 | 59.85 | 207 | 109 |
100.00%
|
100.00%
|
| Primer 38 | Rothia aeria | GGAGGCATCACGCAAGGAT | ATGCGCACATCCAGCTCT | 60.15 | 59.41 | 207 | 109 |
100.00%
|
100.00%
|
| Primer 39 | Rothia aeria | ATCTGTCACCGGTCTGCTC | ATGCACCGAAAGCACCCA | 59.11 | 59.88 | 176 | 108 |
100.00%
|
100.00%
|
| Primer 40 | Rothia aeria | CCTTGCAAGCAGTTCTCGC | TGTGACTCGTTGAGCGCA | 59.79 | 59.58 | 191 | 107 |
100.00%
|
100.00%
|