| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Shigella flexneri | ACATTCTGCTGCTGGGCA | TTGGCCAGTCAGCATCGA | 59.56 | 58.93 | 222 | 109 |
100.00%
|
50.00%
|
| Primer 2 | Shigella flexneri | AGCCCACAAACCGTCGTT | TACGGTTGGCGTGTGCAT | 59.81 | 59.97 | 201 | 89 |
100.00%
|
50.00%
|
| Primer 3 | Shigella flexneri | AGCCCACAAACCGTCGTT | ATACGGTTGGCGTGTGCA | 59.81 | 59.97 | 202 | 78 |
100.00%
|
50.00%
|
| Primer 4 | Shigella flexneri | AAACGTGGACGCCTGGAT | ACCGCTGGCGATCACATT | 59.25 | 59.73 | 171 | 34 |
100.00%
|
50.00%
|