| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 51 | Sinorhizobium fredii | GCGGATGAAATCGCACGAC | GCGAAATGCGACGACGAA | 60.01 | 59.23 | 207 | 73 |
100.00%
|
100.00%
|
| Primer 52 | Sinorhizobium fredii | TGCACGGCAAGATGTCCA | ACCCTGTAACCGATGCAGC | 59.57 | 60.08 | 196 | 71 |
100.00%
|
100.00%
|
| Primer 53 | Sinorhizobium fredii | TGCCGTCAACGCATCGAT | TGCTCGATCGCTGCAAGA | 60.13 | 59.43 | 264 | 70 |
100.00%
|
100.00%
|
| Primer 54 | Sinorhizobium fredii | TGGTCGTCGCCATGCAAA | TGTCGGTCATCCAGATGCG | 60.28 | 59.86 | 261 | 69 |
100.00%
|
100.00%
|
| Primer 55 | Sinorhizobium fredii | TGCGCTTCGGCATCATGA | TTCTTGCGTTCGTCGGTGA | 60.13 | 59.93 | 223 | 68 |
100.00%
|
100.00%
|
| Primer 56 | Sinorhizobium fredii | TCGTGCTTGCCATCTGGA | AGACGTTCAAGCGGTCCTG | 58.93 | 60.01 | 298 | 67 |
100.00%
|
100.00%
|
| Primer 57 | Sinorhizobium fredii | TCATCACCGTGCTTGCCT | TCATCACGCCAACGAGCA | 59.25 | 59.66 | 180 | 67 |
100.00%
|
100.00%
|
| Primer 58 | Sinorhizobium fredii | TCATCATGCTGGCACGGTT | TCATCACGCCAACGAGCA | 60.00 | 59.66 | 237 | 67 |
100.00%
|
100.00%
|
| Primer 59 | Sinorhizobium fredii | TTCGTCGGTTCGCGTGTT | TTTCGTGCGACTCGTGCA | 60.28 | 60.28 | 244 | 67 |
100.00%
|
100.00%
|
| Primer 60 | Sinorhizobium fredii | TTTCGTGCTGCCCTTTGC | AGAAGCTCGATGGCGTTGT | 59.27 | 59.71 | 246 | 66 |
100.00%
|
100.00%
|